Seminar: Approaches to predict small RNA targets in bacteria

By Hakim Tafer, University of Leipzig, Germany. At 15:00 University of Copenhagen, LIFE,  Grønnegårdsvej 3 (bld 1-61) 2nd floor, A211

Registration is not necessary. Refreshments will be served.

Small regulatory RNAs (sRNAs) in eukaryotes and bacteria play an important role in the regulation of gene  expression. They act at the post-transcriptional regulators by base pairing with target mRNAs. Numerous sRNAs have already been identified, but the number of experimentally verified targets is considerably lower. Because experimental verification of putative interactions is a costly and tedious task, in-silico predictions are necessary for functional annotation of  those novel sRNAs. Yet, the computational identification of  these  targets appears to be challenging because frequently  the partial and incomplete sequence complementarity is difficult to evaluate. This talk will review available tools to predict putative  ncRNA targets in bacteria and will then concentrate on two approaches developed in our group, RNAup and RNAplex.