Events

Seminar: Finding the needles in the haystack: The complexity of known and putative novel microRNAs revealed by SOLiD deep sequencing and biology-oriented bioinformatic analysis

By Alessandro Guffanti, Bioinformatics, Genomnia srl - Lainate, Milano, Italy. At 14:30, University of Copenhagen, LIFE, Grønnegårdsvej 7 (bldg. 1-04), 1st floor, Library/M106

Registration is not necessary. Refreshments will be served.

Abstract:

In order to understand microRNA (miRNAs) role in biological systems, it is essential to accurately and comprehensively profile the entire sample miRNA population, and then sail for a long journey toward the uncharted territories of novel small RNA discovery.

This talk will illustrate, going through all the steps of a completed NGS discovery project, how the power of SOLiD deep sequencing coupled with biology-oriented bioinformatic analysis progressively revealed the complexity and variety of the small RNA world in a specific cellular system.

From accurate expression profiling to discovery and primary biological validation, even this limited experiment highlights the sheer amount of non-annotated transcripts in cellular systems which still await detection, quantification and functional annotation.


It also shows how the NGS technology opens the door to a new analytical dimension, where problems and contaminants are as amplified as real discoveries.