Seminar: Tales from the Cellular Underworld: mRNA Decay and Disease
2013-07-04: by Lynne Maquat Lynne E. Maquat, Department of Biochemistry & Biophysics, School of Medicine and Dentistry, Center for RNA Biology, University of Rochester, USA. The Seminar will take place on July 4th, 11.15am -12.00pm at University of Copenhagen, SUND/LIFE, A1-01.15 / Building 1-01, Bülowsvej 17, 1. sal, 1870 Frederiksberg C.
Nonsense-mediated mRNA decay (NMD) and the mechanistically related Staufen1 (STAU1)-mediated mRNA decay (SMD) are critical pathways of cellular post-transcriptional control. NMD generally occurs when translation terminates upstream of an exon-junction complex, and it functions largely as a quality-control pathway that targets newly synthesized mRNA on the cytoplasmic side of the nuclear envelope within 5-to-56 seconds after they emerge from the nucleus. In contrast, SMD occurs when translation terminates upstream of a STAU-binding site (SBS), and it regulates both newly synthesized and steady-state mRNA. New insights into the how the ATP-dependent RNA helicase UPF1, which functions in both pathways, associates with NMD substrates will be provided. Additionally, the roles of STAU1 dimerization, the STAU1 paralog STAU2 and long-noncoding RNAs, which we call a ½-sbsRNAs, in the SMD of human and mouse cells will be discussed. Our studies of SMD in mouse define new functions for lncRNAs and B and identifier (ID) small interspersed elements (SINEs) in myogenesis that undoubtedly also influence many other developmental and homeostatic pathways.