Center for non-coding RNA in Technology and Health (RTH)

The center aims at developing technologies, computational methods as well as experimental approaches for analysis of the mammalian genome for non-coding RNAs in relation to (inflammatory) diseases. The center will focus on developing these technologies to exploit them and the findings in relation to diabetes. The center consists of a number of national and international partners, with the core located at the Faculty for Health and Medical Sciences of University of Copenhagen .

The people in the center cover a range of expertises including computational biology, RNA bioinformatics, molecular models in diabetes, RNA biology, animal models, functional genomics and high-throughput sequence analysis..

Join us


We are always looking for motivated and talented young scientists as well as projects or colaborations within the areas of the center. Feel free to contact us with suggestions or to ask for more information.

News

CRISPRroots

CRISPR roots

2021 December 15. RTH researchers construct new pipeline for post CRISPR-Cas9 assessment of on- and off-targets by combining CRISPR off-target analysis with RNA-seq data and variance calling. The software and the paper are available. The paper was highlighted on CRISPR Medicine News.

Previous news.

Events


We are currently planning more seminars, stay tuned

Previous events.

Recent resources


CRISPRroots

Software

Post editing on and off-target assess­ment with RNAseq data

CRISPRon

Webserver and Software

CRISPR-Cas9 gRNA efficiency prediction webserver based on data integration and deep learning

Conserved RNA Structures in Drosophila

Data Resource

Conserved RNA structures in insect genomes

Research outset


The human genome, made up of DNA, consists of three billion building blocks (nucleotides) where some regions (stretches) are complete genes. We all carry variants of the genes and some cause diseases. Here, the goal is to investigate the specific class of genes, the non-coding RNA genes, in relation to diabetes. The non-coding RNA (ncRNA) genes can be the missing components in diseases that previously have been overlooked.

Our research goal is to develop technologies for ncRNA analysis and to search for functional ncRNAs in relation to diabetes and other (inflammatory) diseases.

Research in details .

Recent publications


CRISPRroots: on- and off-target assessment of RNA-seq data in CRISPR-Cas9 edited cells

Corsi GI, Gadekar VP, Gorodkin J*, Seemann SE* Nucleic Acids Res. 2021 Nov 29
[ PubMed | Paper | Software ]

Astrocytic reactivity triggered by defective autophagy and metabolic failure causes neurotoxicity in frontotemporal dementia type 3

Chandrasekaran A, Dittlau KS, Corsi GI, Haukedal H, Doncheva NT, Ramakrishna S, et al. Stem Cell Reports. 2021 Nov 9;16(11):2736-2751. Epub 2021 Oct 21
[ PubMed | Paper ]