Webservers for CRISPR Cas9 on- and off-target predictions.


State of the art on-target efficiency predictions for CRISPR-Cas9 based on deep learning utilizing the binding energy model developed for CRISPRoff.

Cas9 on-target model

Try the CRISPRon webserver for on-target effiency prediction.


Off-target predictions for CRISPR-Cas9 based on an energy model for the RNA-DNA duplex binding. The model out-performs machine learning models on existing off-target data.

Cas9 off-target model

Try the CRISPRoff webserver to predict CRISPR-Cas9 speci­ficity and off-targets.


Computational pipeline for the analysis of RNA-seq data from CRISPR/Cas9 edited and control cells. The pipeline offers on-target edit verification and detection of possible off-targets affecting the transcripome.


Download the CRISPRroots pipeline here.