CRISPRon(v1.0): CRISPR-Cas9 guide efficiency prediction
This webserver is dedicated to the assessment of guide RNAs for CRISPR-spCas9 applications. Using the webserver, you can compute the on target efficieny of all possible gRNAs with NGG PAM sequences, for genes, genomic regions or custom sequences.
You should also assess the off-target potential of any gRNA before using it in experiments. You can do that from the CRISPRon results page or with the CRISPRoff webserver.
Please fill out the submission form and click the Submit button below.
Citing CRISPRon
If you are using the results of the CRISPRon in your publication, please cite:
CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design
Anthon C, Corsi GI, Gorodkin J*
Bioinformatics. 2022 Dec 13;38(24):5437-5439
[ PubMed | Paper | Webserver ]
Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning
Xiang X†, Corsi GI†, Anthon C†, Qu K†, Pan X, Liang X, Han P, Dong Z, Liu L, Zhong J, Ma T, Wang J, Zhang X, Jiang H, Xu F, Liu X, Xu X, Wang J, Yang H, Bolund L, Church GM, Lin L, Gorodkin J*, Luo Y*
Nat Commun. 2021 May 28;12(1):3238
[ PubMed | Paper | Webserver | Software ]
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