CRISPRon(v1.0): CRISPR-Cas9 guide efficiency prediction

This webserver is dedicated to the assessment of guide RNAs for CRISPR-spCas9 applications. Using the webserver, you can compute the on target efficieny of all possible gRNAs with NGG PAM sequences, for genes, genomic regions or custom sequences.

You should also assess the off-target potential of any gRNA before using it in experiments. You can do that with the CRISPRoff webserver.

Please fill out the submission form and click the Submit button below.

Job options


Fill in a genome assembly and a query sequence or region and press submit below.

(When you press submit, you accept that your data and E-mail are stored on our servers for up to 14 days for the sole purpose of processing your job and reporting back to you.)

Citing CRISPRon


If you are using the results of the CRISPRon in your publication, please cite:

Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning

Xiang X, Corsi GI, Anthon C, Qu K, Pan X, Liang X, Han P, Dong Z, Liu L, Zhong J, Ma T, Wang J, Zhang X, Jiang H, Xu F, Liu X, Xu X, Wang J, Yang H, Bolund L, Church GM, Lin L, Gorodkin J*, Luo Y* Nature Communications 2021, 12(1)
[ Paper ]

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