CRISPRoff (v1.2beta): off-targeting assessment of Cas9 gRNAs

This webserver is dedicated to the assessment of guide RNAs for CRISPR-spCas9 applications. Using the webserver, you can compute the specificity of gRNAs with NGG PAM sequences. You will also obtain the relative likelyhood of cleavage by CRISPR-Cas9 on off-target sites compared to the likelyhood of cleavage at the on-target site. Off-targets with up to 6 mis-matches for the gRNA and NGG, NGA and NAG PAM sequences are considered by the webserver.

You should also assess the on-target effeciency of any gRNA before using it in experiments. You can do that with the CRISPRon webserver.

Job options

Fill in a genome assembly and a query sequence and press submit below.


Load examples (different styles):  gRNA (single, multiple)  design gRNAs for target sequence (single, multiple)

(When you press submit, you accept that your data and E-mail are stored on our servers for up to 14 days for the sole purpose of processing your job and reporting back to you.)

References

CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters

Alkan F, Wenzel A, Anthon C, Havgaard JH, Gorodkin J Genome Biol. 2018 Oct 26;19(1):177
[ PubMed | Paper | Webserver | Software ]

RIsearch2: suffix array-based large-scale prediction of RNA-RNA interactions and siRNA off-targets

Alkan F, Wenzel A, Palasca O, Kerpedjiev P, Rudebeck AF, Stadler PF, Hofacker IL, Gorodkin J Nucleic Acids Res. 2017 May 5;45(8):e60
[ PubMed | Paper | Software ]

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