CRISPR-OFF webserver (v1.1): off-targeting assessment of Cas9 gRNAs

Downloads


For the stand-alone version of CRISPRspec and CRISPRoff computing pipeline, you can download the python scripts that are used in the background of our webserver in the following link. The pipeline is released under the the GNU public license version 3 or later.


Additionally, to perform off-target predictions in the organisms that are not included in this webserver, you can access the RIsearch2(v2.1) RNA-RNA interaction prediction software, that can localize Cas9-off-targets (v2.1 or higher), by clicking here.

Other sources


To perform off-target predictions in the organisms that are not included in this webserver, we highly recommend to use RIsearch2 (v2.1) RNA-RNA interaction prediction software, that can also localize Cas9-off-targets (v2.1). Read our About page for more details on which parameters to use when running RIsearch2.

Cas-OFFinder webserver can also help with off-target predictions in the organisms that are not included in this webserver. You can upload CasOFFinder result files to our webserver to perform the off-targeting assessment of your gRNAs with CRISPRoff and CRISPRspec scores.


References


CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters
Alkan F, Wenzel A, Anthon C, Havgaard JH, Gorodkin J Genome Biol. 2018 Oct 26;19(1):177
[ PubMed | Paper | Webserver | Software ]

Feedback


We greatly appreciate your comments. Open Feedback form in a new tab.

Please contact us for your questions and comments.
ferro@rth.dk
gorodkin@rth.dk