CRISPR-OFF webserver (v1.1): off-targeting assessment of Cas9 gRNAs

This webserver is dedicated to the off-targeting assessment of different gRNA/on-target selections for CRISPR-Cas9 applications. Using the webserver, you can compute the CRISPRoff off-target confidence scores of off-target predictions of a gRNA and you can compare different gRNA/on-target selections using the CRISPRspec specificity score.

Please fill out the submission form and click the Submit button given below. Do NOT forget to enter your email address.

INPUT-1: gRNA / on-target sequence(s)

Sequence in plain or fasta format, they are both accepted. (gRNAs will always be 23nts, inluding the PAM NGG)
Load examples (different styles):  gRNA (single, multiple)  design gRNAs for target sequence (single, multiple)


(Max 100 gRNAs, 2300 nucleotides)

INPUT-2: off-target predictions

Would you like the webserver to perform the off-target predictions?

Enter your e-mail adress to receive a notification after the job has finished, and optionally give your job any custom name.
E-mail    Job name   

References


CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters
Alkan F, Wenzel A, Anthon C, Havgaard JH, Gorodkin J Genome Biol. 2018 Oct 26;19(1):177
[ PubMed | Paper | Webserver | Software ]

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Please contact us for your questions and comments.
ferro@rth.dk
gorodkin@rth.dk