sigM
BSGatlas-gene-1154
BSGatlas
Description | Information |
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Coordinates | 1029577..1030068 |
Genomic Size | 492 bp |
Name | sigM |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | - |
Type | CDS |
SubtiWiki
Description | Information |
---|---|
Alternative Name | sigM |
sigM | |
yhdM | |
Category | SW 3 Information processing |
SW 3.2 RNA synthesis and degradation | |
SW 3.2.1 Transcription | |
SW 3.2.1.2 Sigma factors | |
SW 3.4 Regulation of gene expression | |
SW 3.4.1 Sigma factors and their control | |
SW 3.4.1.1 Sigma factors | |
SW 4 Lifestyles | |
SW 4.3 Coping with stress | |
SW 4.3.13 Resistance against toxins/ antibiotics | |
SW 4.3.2 Cell envelope stress proteins (controlled by SigM, V, W, X, Y) | |
Description | [SW|RNA polymerase] ECF-type [SW|sigma factor] [[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41]], required for adaptation to inhibitors of peptidoglycan synthesis |
Function | adaptation to inhibitors of peptidoglycan synthesis |
Is essential? | no |
Isoelectric point | 6.77 |
Locus Tag | BSU_09520 |
Molecular weight | 19.2635 |
Name | sigM |
Product | [SW|RNA polymerase] ECF-type [SW|sigma factor] [[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41]] |
RefSeq
Description | Information |
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Alternative Locus Tag | BSU09520 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 14651641, 14769884;Product type r: regulator |
Functions | 16.3: Control |
Locus Tag | BSU_09520 |
Name | sigM |
Title | RNA polymerase ECF (extracytoplasmicfunction)-type sigma factor (sigma(M)) |
Type | CDS |
BsubCyc
Description | Information |
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Alternative Name | yhdM |
Citation | Hashimoto M;Seki T;Matsuoka S;Hara H;Asai K;Sadaie Y;Matsumoto K Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis. Microbiology 159(Pt 1);23-35 (2013) PUBMED: 23103977 |
Helmann JD Bacillus subtilis extracytoplasmic function (ECF) sigma factors and defense of the cell envelope. Curr Opin Microbiol 30;122-132 (2016) PUBMED: 26901131 | |
Inoue H;Suzuki D;Asai K A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO. Biochem Biophys Res Commun 436(1);6-11 (2013) PUBMED: 23632331 | |
Kingston AW;Liao X;Helmann JD Contributions of the σ(W) , σ(M) and σ(X) regulons to the lantibiotic resistome of Bacillus subtilis. Mol Microbiol 90(3);502-18 (2013) PUBMED: 23980836 | |
Luo Y;Asai K;Sadaie Y;Helmann JD Transcriptomic and phenotypic characterization of a Bacillus subtilis strain without extracytoplasmic function sigma factors. J Bacteriol 192(21);5736-45 (2010) PUBMED: 20817771 | |
Luo Y;Helmann JD Analysis of the role of Bacillus subtilis σ(M) in β-lactam resistance reveals an essential role for c-di-AMP in peptidoglycan homeostasis. Mol Microbiol 83(3);623-39 (2012) PUBMED: 22211522 | |
Ogura M;Asai K Glucose Induces ECF Sigma Factor Genes, sigX and sigM, Independent of Cognate Anti-sigma Factors through Acetylation of CshA in Bacillus subtilis. Front Microbiol 7;1918 (2016) PUBMED: 27965645 | |
Seki T;Mineshima R;Hashimoto M;Matsumoto K;Hara H;Matsuoka S Repression of the activities of two extracytoplasmic function σ factors, σ(M) and σ(V), of Bacillus subtilis by glucolipids in Escherichia coli cells. Genes Genet Syst 90(2);109-14 (2015) PUBMED: 26399770 | |
Souza BM;Castro TL;Carvalho RD;Seyffert N;Silva A;Miyoshi A;Azevedo V σ(ECF) factors of gram-positive bacteria: a focus on Bacillus subtilis and the CMNR group. Virulence 5(5);587-600 (2014) PUBMED: 24921931 | |
Wecke T;Bauer T;Harth H;Mader U;Mascher T The rhamnolipid stress response of Bacillus subtilis. FEMS Microbiol Lett 323(2);113-23 (2011) PUBMED: 22092710 | |
Comment | 16.3: Control |
Description | RNA polymerase ECF (extracytoplasmic function)-type sigma factor (sigma(M)) |
Gene Ontology | GO:0003677 DNA binding |
GO:0003700 DNA-binding transcription factor activity | |
GO:0006351 transcription, DNA-templated | |
GO:0006352 DNA-templated transcription, initiation | |
GO:0006355 regulation of transcription, DNA-templated | |
GO:0016987 sigma factor activity | |
Locus Tag | BSU09520 |
Molecular weight | 19.407 |
Name | sigM |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | BSU14930, BSU25190, BSU14920, BSU14900, BSU14890, BSU23450, BSU22540, BSU14910, BSU_misc_RNA_1, BSU_misc_RNA_3, BSU_misc_RNA_6, BSU_misc_RNA_7, BSU_misc_RNA_8, BSU_misc_RNA_9, BSU_misc_RNA_10, BSU_misc_RNA_11, BSU_misc_RNA_12, BSU_misc_RNA_13, BSU_misc_RNA_14, BSU_misc_RNA_15, BSU_misc_RNA_16, BSU_misc_RNA_17, BSU_misc_RNA_18, BSU_misc_RNA_19, BSU_misc_RNA_20, BSU_misc_RNA_21, BSU_misc_RNA_23, BSU_misc_RNA_24, BSU_misc_RNA_25, BSU_misc_RNA_26, BSU_misc_RNA_27, BSU_misc_RNA_28, BSU_misc_RNA_29, BSU_misc_RNA_31, BSU_misc_RNA_32, BSU_misc_RNA_34, BSU_misc_RNA_36, BSU_misc_RNA_38, BSU_misc_RNA_39, BSU_misc_RNA_40, BSU_misc_RNA_41, BSU_misc_RNA_42, BSU_misc_RNA_44, BSU_misc_RNA_45, BSU_misc_RNA_46, BSU_misc_RNA_47, BSU_misc_RNA_48, BSU_misc_RNA_49, BSU_misc_RNA_50, BSU_misc_RNA_51, BSU_misc_RNA_52, BSU_misc_RNA_53, BSU_misc_RNA_54, BSU_misc_RNA_56, BSU_misc_RNA_59, BSU_misc_RNA_60, BSU_misc_RNA_63, BSU19030 |
Expression pos. correlated with | BSU09510, BSU38120, BSU04230, new_3694080_3694200, new_1030069_1030171_c, new_2305261_2305347, new_2349529_2349600_c, BSU22390, new_2339954_2340289, BSU38499 |
Highly expressed condition | (Cold) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. |
(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(H2O2) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(Heat) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(LBGstat) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(Paraq) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(Salt) Cells were grown in Spizizen’s minimal medium (SMM) at 37 °C with vigorous shaking. Salt was added, to a final concentration of 0.4 M to an exponentially growing culture of cells at OD500 of 0.4. Samples were harvested before [SMM] and 10 minutes after [Salt] NaCl addition. | |
(T-0.40H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T-1.10H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T0.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Lowely expressed condition | (BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(M0t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(M40t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(S2) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S3) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
Name | sigM |