kinA
BSGatlas-gene-1665
BSGatlas
Description | Information |
---|---|
Coordinates | 1470026..1471846 |
Genomic Size | 1821 bp |
Name | kinA |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | + |
Type | CDS |
SubtiWiki
Description | Information |
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Alternative Name | gsiC |
kinA | |
kinA | |
scoD | |
spoIIF | |
spoIIJ | |
Category | SW 3 Information processing |
SW 3.3 Protein synthesis, modification and degradation | |
SW 3.3.4 Protein modification | |
SW 3.3.4.2 Protein kinases | |
SW 3.4 Regulation of gene expression | |
SW 3.4.2 Transcription factors and their control | |
SW 3.4.2.2 Control of two-component response regulators | |
SW 3.4.2.2.1 Two-component sensor kinase | |
SW 3.4.7 phosphorelay | |
SW 3.4.7.1 The kinases | |
SW 4 Lifestyles | |
SW 4.2 Sporulation | |
SW 4.2.2 phosphorelay | |
SW 4.2.2.1 The kinases | |
SW 6 Groups of genes | |
SW 6.4 Phosphoproteins | |
SW 6.4.4 Phosphorylation on a His residue | |
Description | two-component sensor kinase, phosphorylates [[protein|9896B99346B0D3F6D57F57377DB253B46135A37A]], part of the [SW|phosphorelay] |
Function | initiation of sporulation |
Is essential? | no |
Isoelectric point | 5.49 |
Locus Tag | BSU_13990 |
Molecular weight | 68.9945 |
Name | kinA |
Product | two-component sensor kinase |
RefSeq
Description | Information |
---|---|
Alternative Locus Tag | BSU13990 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 11734624, 14629015,17350039, 21097618, 22303282, 23169620, 23378509,23504013, 26712348, 28449380; Product type e: enzyme |
Enzyme Classifications | EC 2.7.13.3: histidine kinase |
Functions | 16.12: Sense |
16.3: Control | |
Locus Tag | BSU_13990 |
Name | kinA |
Title | sporulation-specific ATP-dependent proteinhistidine kinase |
Type | CDS |
BsubCyc
Description | Information |
---|---|
Alternative Name | gsiC |
scoB | |
scoD | |
spoIIF | |
spoIIJ | |
Citation | Boguslawski KM;Hill PA;Griffith KL Novel mechanisms of controlling the activities of the transcription factors Spo0A and ComA by the plasmid-encoded quorum sensing regulators Rap60-Phr60 in Bacillus subtilis. Mol Microbiol 96(2);325-48 (2015) PUBMED: 25598361 |
Dago AE;Schug A;Procaccini A;Hoch JA;Weigt M;Szurmant H Structural basis of histidine kinase autophosphorylation deduced by integrating genomics, molecular dynamics, and mutagenesis. Proc Natl Acad Sci U S A 109(26);E1733-42 (2012) PUBMED: 22670053 | |
Devi SN;Kiehler B;Haggett L;Fujita M Evidence that autophosphorylation of the major sporulation kinase in Bacillus subtilis is able to occurs in trans. J Bacteriol (2015) PUBMED: 26055117 | |
Eswaramoorthy P;Dravis A;Devi SN;Vishnoi M;Dao HA;Fujita M Expression level of a chimeric kinase governs entry into sporulation in Bacillus subtilis. J Bacteriol 193(22);6113-22 (2011) PUBMED: 21926229 | |
Eswaramoorthy P;Duan D;Dinh J;Dravis A;Devi SN;Fujita M The threshold level of the sensor histidine kinase KinA governs entry into sporulation in Bacillus subtilis. J Bacteriol 192(15);3870-82 (2010) PUBMED: 20511506 | |
Kolodkin-Gal I;Elsholz AK;Muth C;Girguis PR;Kolter R;Losick R Respiration control of multicellularity in Bacillus subtilis by a complex of the cytochrome chain with a membrane-embedded histidine kinase. Genes Dev 27(8);887-99 (2013) PUBMED: 23599347 | |
McLoon AL;Kolodkin-Gal I;Rubinstein SM;Kolter R;Losick R Spatial regulation of histidine kinases governing biofilm formation in Bacillus subtilis. J Bacteriol 193(3);679-85 (2011) PUBMED: 21097618 | |
Narula J;Devi SN;Fujita M;Igoshin OA Ultrasensitivity of the Bacillus subtilis sporulation decision. Proc Natl Acad Sci U S A 109(50);E3513-22 (2012) PUBMED: 23169620 | |
Tojo S;Hirooka K;Fujita Y Expression of kinA and kinB of Bacillus subtilis, Necessary for Sporulation Initiation, Is under Positive Stringent Transcription Control. J Bacteriol 195(8);1656-65 (2013) PUBMED: 23378509 | |
Winnen B;Anderson E;Cole JL;King GF;Rowland SL Role of the PAS sensor domains in the Bacillus subtilis sporulation kinase KinA. J Bacteriol 195(10);2349-58 (2013) PUBMED: 23504013 | |
Comment | Note that |CITS: [20391342]| has been retracted |CITS: [21968764]|. 16.3: Control 16.12: Sense |
Description | sporulation-specific ATP-dependent protein histidine kinase |
Gene Ontology | GO:0000155 phosphorelay sensor kinase activity |
GO:0000160 phosphorelay signal transduction system | |
GO:0000166 nucleotide binding | |
GO:0004673 protein histidine kinase activity | |
GO:0004871 NA | |
GO:0005515 protein binding | |
GO:0005524 ATP binding | |
GO:0006355 regulation of transcription, DNA-templated | |
GO:0007165 signal transduction | |
GO:0016020 membrane | |
GO:0016301 kinase activity | |
GO:0016310 phosphorylation | |
GO:0016740 transferase activity | |
GO:0016772 transferase activity, transferring phosphorus-containing groups | |
GO:0018106 peptidyl-histidine phosphorylation | |
GO:0023014 signal transduction by protein phosphorylation | |
GO:0030435 sporulation resulting in formation of a cellular spore | |
GO:0042802 identical protein binding | |
GO:0043938 positive regulation of sporulation | |
Locus Tag | BSU13990 |
Molecular weight | 69.171 |
Name | kinA |
Nicolas et al. predictions
Description | Information |
---|---|
Expression neg. correlated with | BSU14630, new_967323_967934, BSU07430, BSU40440, BSU25500, BSU08910, BSU27610, BSU03610, BSU03600, BSU03590 |
Expression pos. correlated with | new_2116747_2116967_c, BSU10750, BSU13530, new_2829529_2830447_c, BSU13070, BSU18370, BSU18360, new_1153150_1153230, BSU10180, BSU13080 |
Highly expressed condition | (Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. |
(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(M0t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(S1) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S2) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T2.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T3.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T3.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T4.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T5.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Lowely expressed condition | (C30) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal. |
(dia5) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15]. | |
(Diami) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(G135) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(G150) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(G180) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(Heat) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(LBGexp) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
Name | kinA |
KEGG Pathways
Description | Information |
---|---|
Pathway | Two-component system (ko02020) |