kinC
BSGatlas-gene-1719
BSGatlas
Description | Information |
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Coordinates | 1518333..1519619 |
Genomic Size | 1287 bp |
Name | kinC |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | + |
Type | CDS |
SubtiWiki
Description | Information |
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Alternative Name | kinC |
Category | SW 3 Information processing |
SW 3.3 Protein synthesis, modification and degradation | |
SW 3.3.4 Protein modification | |
SW 3.3.4.2 Protein kinases | |
SW 3.4 Regulation of gene expression | |
SW 3.4.2 Transcription factors and their control | |
SW 3.4.2.2 Control of two-component response regulators | |
SW 3.4.2.2.1 Two-component sensor kinase | |
SW 3.4.7 phosphorelay | |
SW 3.4.7.1 The kinases | |
SW 4 Lifestyles | |
SW 4.1 Exponential and early post-exponential lifestyles | |
SW 4.1.2 Biofilm formation | |
SW 4.1.2.4 Regulation | |
SW 4.2 Sporulation | |
SW 4.2.2 phosphorelay | |
SW 4.2.2.1 The kinases | |
SW 6 Groups of genes | |
SW 6.2 Membrane proteins | |
SW 6.4 Phosphoproteins | |
SW 6.4.4 Phosphorylation on a His residue | |
Description | two-component sensor kinase, phosphorylates [[protein|9896B99346B0D3F6D57F57377DB253B46135A37A]] and [[protein|2C54FE2ADC82FF414D732018C90649D477A925AD]], part of the [SW|phosphorelay], governs expression of genes involved in [SW|biofilm formation] |
Function | initiation of [SW|sporulation] |
Is essential? | no |
Isoelectric point | 6.22 |
Locus Tag | BSU_14490 |
Molecular weight | 48.6824 |
Name | kinC |
Product | two-component sensor kinase |
RefSeq
Description | Information |
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Alternative Locus Tag | BSU14490 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 11069677, 11673427,7836330, 20971918, 25701730, 26152584, 23569226, 26297017;Product type e: enzyme |
Enzyme Classifications | EC 2.7.13.3: histidine kinase |
Functions | 16.3: Control |
Locus Tag | BSU_14490 |
Name | kinC |
Title | two-component sensor potassium-responsivehistidine kinase regulating cannibalism and biofilmformation |
Type | CDS |
BsubCyc
Description | Information |
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Alternative Name | ssb |
Citation | Devi SN;Vishnoi M;Kiehler B;Haggett L;Fujita M In vivo functional characterization of the transmembrane histidine kinase KinC in Bacillus subtilis. Microbiology 161(Pt 5);1092-104 (2015) PUBMED: 25701730 |
Grau RR;de Ona P;Kunert M;Lenini C;Gallegos-Monterrosa R;Mhatre E;Vileta D;Donato V;Holscher T;Boland W;Kuipers OP;Kovacs ÁT A Duo of Potassium-Responsive Histidine Kinases Govern the Multicellular Destiny of Bacillus subtilis. MBio 6(4);e00581 (2015) PUBMED: 26152584 | |
Lopez D;Gontang EA;Kolter R Potassium Sensing Histidine Kinase in Bacillus subtilis. Methods Enzymol 471;229-51 (2010) PUBMED: 20946851 | |
Lopez D Connection of KinC to flotillins and potassium leakage in Bacillus subtilis. Microbiology 161(6);1180-1 (2015) PUBMED: 25934647 | |
Lundberg ME;Becker EC;Choe S MstX and a Putative Potassium Channel Facilitate Biofilm Formation in Bacillus subtilis. PLoS One 8(5);e60993 (2013) PUBMED: 23737939 | |
McLoon AL;Kolodkin-Gal I;Rubinstein SM;Kolter R;Losick R Spatial regulation of histidine kinases governing biofilm formation in Bacillus subtilis. J Bacteriol 193(3);679-85 (2011) PUBMED: 21097618 | |
Schneider J;Mielich-Suss B;Bohme R;Lopez D In vivo characterization of the scaffold activity of flotillin on the membrane kinase KinC of Bacillus subtilis. Microbiology 161(9);1871-87 (2015) PUBMED: 26297017 | |
Shemesh M;Kolter R;Losick R The biocide chlorine dioxide stimulates biofilm formation in Bacillus subtilis by activation of the histidine kinase KinC. J Bacteriol 192(24);6352-6 (2010) PUBMED: 20971918 | |
Vishnoi M;Narula J;Devi SN;Dao HA;Igoshin OA;Fujita M Triggering sporulation in Bacillus subtilis with artificial two-component systems reveals the importance of proper Spo0A activation dynamics. Mol Microbiol 90(1);181-94 (2013) PUBMED: 23927765 | |
Comment | 16.3: Control |
Description | two-component sensor histidine kinase |
Gene Ontology | GO:0000155 phosphorelay sensor kinase activity |
GO:0000160 phosphorelay signal transduction system | |
GO:0000166 nucleotide binding | |
GO:0004673 protein histidine kinase activity | |
GO:0004871 NA | |
GO:0005524 ATP binding | |
GO:0005886 plasma membrane | |
GO:0006355 regulation of transcription, DNA-templated | |
GO:0007165 signal transduction | |
GO:0016020 membrane | |
GO:0016021 integral component of membrane | |
GO:0016301 kinase activity | |
GO:0016310 phosphorylation | |
GO:0016740 transferase activity | |
GO:0016772 transferase activity, transferring phosphorus-containing groups | |
GO:0018106 peptidyl-histidine phosphorylation | |
GO:0023014 signal transduction by protein phosphorylation | |
GO:0030435 sporulation resulting in formation of a cellular spore | |
Locus Tag | BSU14490 |
Molecular weight | 48.846 |
Name | kinC |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | BSU27450, BSU24170, BSU24150, BSU27460, BSU18210, BSU24160, BSU15760, BSU24140, new_4010329_4010403, BSU24130, BSU18220 |
Expression pos. correlated with | BSU14500, BSU06090, BSU34710, BSU34720, BSU35480, BSU29610, new_3566276_3566357_c, BSU10650, BSU27650, BSU40970 |
Highly expressed condition | (Cold) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. |
(dia0) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15]. | |
(LBGtran) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LBtran) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(M0t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(M0t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(M40t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(M40t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(T0.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Lowely expressed condition | (B36) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(B60) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. | |
(LoTm) Cells were grown in Spizizen’s minimal medium (SMM) (C. Anagnostopoulos, J. Spizizen, J Bacteriol 81, 741, May, 1961) with vigorous agitation. The control culture was grown at 37 °C [SMMPr]. For growth at high or low temperatures, pre-cultures were grown at 37 °C, diluted to an OD578nm of 0.1 and subsequently transferred to 51 °C [HiTm] and 16 °C [LoTm], respectively. For the growth at high salinity, the salinity of the medium was adjusted by adding NaCl (5 M stock solution) to produce a final concentration of 1.2 M [HiOs]. | |
(S3) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T5.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Name | kinC |
KEGG Pathways
Description | Information |
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Pathway | Two-component system (ko02020) |