ftsZ
BSGatlas-gene-1808
BSGatlas
Description | Information |
---|---|
Coordinates | 1597832..1598980 |
Genomic Size | 1149 bp |
Name | ftsZ |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | + |
Type | CDS |
SubtiWiki
Description | Information |
---|---|
Alternative Name | ftsZ |
ftsZ | |
ts-1 | |
Category | SW 1 Cellular processes |
SW 1.1 Cell envelope and cell division | |
SW 1.1.8 Cell division | |
SW 1.1.8.2 Other genes | |
SW 6 Groups of genes | |
SW 6.1 Essential genes | |
SW 6.2 Membrane proteins | |
Description | cell-division initiation protein (septum formation) |
Function | formation of Z-ring |
Is essential? | yes |
Isoelectric point | 4.81 |
Locus Tag | BSU_15290 |
Molecular weight | 40.1954 |
Name | ftsZ |
Product | cell-division initiation protein (septum formation), member of the [SW|divisome] |
RefSeq
Description | Information |
---|---|
Alternative Locus Tag | BSU15290 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 12682299, 12950927,14527275, 15317782, 16159787, 16322744, 16549676,22720735; Product type cp: cell process |
Functions | 16.13: Shape |
Locus Tag | BSU_15290 |
Name | ftsZ |
Title | cell-division initiation protein |
Type | CDS |
BsubCyc
Description | Information |
---|---|
Alternative Name | ts-1 |
Citation | Adams DW;Wu LJ;Czaplewski LG;Errington J Multiple effects of benzamide antibiotics on FtsZ function. Mol Microbiol 80(1);68-84 (2011) PUBMED: 21276094 |
Araujo-Bazan L;Ruiz-Avila LB;Andreu D;Huecas S;Andreu JM Cytological Profile of Antibacterial FtsZ Inhibitors and Synthetic Peptide MciZ. Front Microbiol 7;1558 (2016) PUBMED: 27752253 | |
Artola M;Ruiz-Avila LB;Vergonos A;Huecas S;Araujo-Bazan L;Martin-Fontecha M;Vazquez-Villa H;Turrado C;Ramirez-Aportela E;Hoegl A;Nodwell M;Barasoain I;Chacon P;Sieber SA;Andreu JM;Lopez-Rodriguez ML Effective GTP-replacing FtsZ inhibitors and antibacterial mechanism of action. ACS Chem Biol 10(3);834-43 (2015) PUBMED: 25486266 | |
Bhattacharya A;Jindal B;Singh P;Datta A;Panda D Plumbagin inhibits cytokinesis in Bacillus subtilis by inhibiting FtsZ assembly - a mechanistic study of its antibacterial activity. FEBS J 280(18);4585-99 (2013) PUBMED: 23841620 | |
Bi F;Guo L;Wang Y;Venter H;Semple SJ;Liu F;Ma S Design, synthesis and biological activity evaluation of novel 2,6-difluorobenzamide derivatives through FtsZ inhibition. Bioorg Med Chem Lett 27(4);958-962 (2017) PUBMED: 28082038 | |
Blasios V;Bisson-Filho AW;Castellen P;Nogueira ML;Bettini J;Portugal RV;Zeri AC;Gueiros-Filho FJ Genetic and Biochemical Characterization of the MinC-FtsZ Interaction in Bacillus subtilis. PLoS One 8(4);e60690 (2013) PUBMED: 23577149 | |
Buske PJ;Levin PA A flexible C-terminal linker is required for proper FtsZ assembly in vitro and cytokinetic ring formation in vivo. Mol Microbiol 89(2);249-63 (2013) PUBMED: 23692518 | |
Buske PJ;Levin PA Extreme C Terminus of Bacterial Cytoskeletal Protein FtsZ Plays Fundamental Role in Assembly Independent of Modulatory Proteins. J Biol Chem 287(14);10945-57 (2012) PUBMED: 22298780 | |
de Oliveira IF;de Sousa Borges A;Kooij V;Bartosiak-Jentys J;Luirink J;Scheffers DJ Characterization of ftsZ mutations that render Bacillus subtilis resistant to MinC. PLoS One 5(8);e12048 (2010) PUBMED: 20711458 | |
Dhaked HP;Bhattacharya A;Yadav S;Dantu SC;Kumar A;Panda D Mutation of Arg191 in FtsZ Impairs Cytokinetic Abscission of Bacillus subtilis Cells. Biochemistry 55(40);5754-5763 (2016) PUBMED: 27629358 | |
Dichosa AE;Fitzsimons MS;Lo CC;Weston LL;Preteska LG;Snook JP;Zhang X;Gu W;McMurry K;Green LD;Chain PS;Detter JC;Han CS Artificial polyploidy improves bacterial single cell genome recovery. PLoS One 7(5);e37387 (2012) PUBMED: 22666352 | |
Foss MH;Eun YJ;Grove CI;Pauw DA;Sorto NA;Rensvold JW;Pagliarini DJ;Shaw JT;Weibel DB Inhibitors of bacterial tubulin target bacterial membranes in vivo. Medchemcomm 4(1);112-119 (2013) PUBMED: 23539337 | |
Groundwater PW;Narlawar R;Liao VW;Bhattacharya A;Srivastava S;Kunal K;Doddareddy M;Oza PM;Mamidi R;Marrs EC;Perry JD;Hibbs DE;Panda D A Carbocyclic Curcumin Inhibits Proliferation of Gram-Positive Bacteria by Targeting FtsZ. Biochemistry 56(3);514-524 (2017) PUBMED: 28002947 | |
Huecas S;Marcelo F;Perona A;Ruiz-Avila LB;Morreale A;Canada FJ;Jimenez-Barbero J;Andreu JM Beyond a Fluorescent Probe: Inhibition of Cell Division Protein FtsZ by mant-GTP Elucidated by NMR and Biochemical Approaches. ACS Chem Biol 10(10);2382-92 (2015) PUBMED: 26247422 | |
Jindal B;Panda D Understanding FtsZ assembly: cues from the behavior of its N- and C-terminal domains. Biochemistry 52(40);7071-81 (2013) PUBMED: 24007276 | |
Krol E;de Sousa Borges A;da Silva I;Polaquini CR;Regasini LO;Ferreira H;Scheffers DJ Antibacterial activity of alkyl gallates is a combination of direct targeting of FtsZ and permeabilization of bacterial membranes. Front Microbiol 6;390 (2015) PUBMED: 25972861 | |
Krol E;Scheffers DJ FtsZ polymerization assays: simple protocols and considerations. J Vis Exp (81);e50844 (2013) PUBMED: 24300445 | |
Krol E;van Kessel SP;van Bezouwen LS;Kumar N;Boekema EJ;Scheffers DJ Bacillus subtilis SepF Binds to the C-Terminus of FtsZ. PLoS One 7(8);e43293 (2012) PUBMED: 22912848 | |
Loi VV;Rossius M;Antelmann H Redox regulation by reversible protein S-thiolation in bacteria. Front Microbiol 6;187 (2015) PUBMED: 25852656 | |
Marcelo F;Huecas S;Ruiz-Avila LB;Canada FJ;Perona A;Poveda A;Martin-Santamaria S;Morreale A;Jimenez-Barbero J;Andreu JM Interactions of bacterial cell division protein FtsZ with C8-substituted guanine nucleotide inhibitors. A combined NMR, biochemical and molecular modeling perspective. J Am Chem Soc 135(44);16418-28 (2013) PUBMED: 24079270 | |
Monahan LG;Hajduk IV;Blaber SP;Charles IG;Harry EJ Coordinating bacterial cell division with nutrient availability: a role for glycolysis. MBio 5(3);e00935-14 (2014) PUBMED: 24825009 | |
Monahan LG;Harry EJ You Are What You Eat: Metabolic Control of Bacterial Division. Trends Microbiol 24(3);181-9 (2016) PUBMED: 26690613 | |
Monahan LG;Liew AT;Bottomley AL;Harry EJ Division site positioning in bacteria: one size does not fit all. Front Microbiol 5;19 (2014) PUBMED: 24550892 | |
Monahan LG;Robinson A;Harry EJ Lateral FtsZ association and the assembly of the cytokinetic Z ring in bacteria. Mol Microbiol 74(4);1004-17 (2009) PUBMED: 19843223 | |
Randich AM;Brun YV Molecular mechanisms for the evolution of bacterial morphologies and growth modes. Front Microbiol 6;580 (2015) PUBMED: 26106381 | |
Ray S;Dhaked HP;Panda D Antimicrobial peptide CRAMP (16-33) stalls bacterial cytokinesis by inhibiting FtsZ assembly. Biochemistry 53(41);6426-9 (2014) PUBMED: 25294259 | |
Ray S;Jindal B;Kunal K;Surolia A;Panda D BT-benzo-29 inhibits bacterial cell proliferation by perturbing FtsZ assembly. FEBS J 282(20);4015-33 (2015) PUBMED: 26258635 | |
Ray S;Kumar A;Panda D GTP regulates the interaction between MciZ and FtsZ: a possible role of MciZ in bacterial cell division. Biochemistry 52(2);392-401 (2013) PUBMED: 23237472 | |
Ruiz-Avila LB;Huecas S;Artola M;Vergonos A;Ramirez-Aportela E;Cercenado E;Barasoain I;Vazquez-Villa H;Martin-Fontecha M;Chacon P;Lopez-Rodrı́guez ML;Andreu JM Synthetic Inhibitors of Bacterial Cell Division Targeting the GTP-Binding Site of FtsZ. ACS Chem Biol 8(9);2072-2083 (2013) PUBMED: 23855511 | |
Santini T;Turchi L;Ceccarelli G;Di Franco C;Beccari E Transcriptional analysis of ftsZ within the dcw cluster in Bacillus mycoides. BMC Microbiol 13(1);27 (2013) PUBMED: 23384289 | |
Singh D;Bhattacharya A;Rai A;Dhaked HP;Awasthi D;Ojima I;Panda D SB-RA-2001 Inhibits Bacterial Proliferation by Targeting FtsZ Assembly. Biochemistry 53(18);2979-92 (2014) PUBMED: 24749867 | |
Strauss MP;Liew AT;Turnbull L;Whitchurch CB;Monahan LG;Harry EJ 3D-SIM Super Resolution Microscopy Reveals a Bead-Like Arrangement for FtsZ and the Division Machinery: Implications for Triggering Cytokinesis. PLoS Biol 10(9);e1001389 (2012) PUBMED: 22984350 | |
Turnbull L;Strauss MP;Liew AT;Monahan LG;Whitchurch CB;Harry EJ Super-resolution imaging of the cytokinetic Z ring in live bacteria using fast 3D-structured illumination microscopy (f3D-SIM). J Vis Exp (91);51469 (2014) PUBMED: 25286090 | |
Wu LJ;Errington J Nucleoid occlusion and bacterial cell division. Nat Rev Microbiol 10(1);8-12 (2012) PUBMED: 22020262 | |
Comment | 16.13: Shape |
Description | cell-division initiation protein |
Gene Ontology | GO:0000166 nucleotide binding |
GO:0000917 division septum assembly | |
GO:0003924 GTPase activity | |
GO:0005515 protein binding | |
GO:0005525 GTP binding | |
GO:0005737 cytoplasm | |
GO:0007049 cell cycle | |
GO:0030428 cell septum | |
GO:0032153 cell division site | |
GO:0042802 identical protein binding | |
GO:0043093 FtsZ-dependent cytokinesis | |
GO:0043234 NA | |
GO:0051258 protein polymerization | |
GO:0051301 cell division | |
Locus Tag | BSU15290 |
Molecular weight | 40.395 |
Name | ftsZ |
Nicolas et al. predictions
Description | Information |
---|---|
Expression neg. correlated with | new_3421635_3422223_c, BSU35040, BSU17770, BSU02150, BSU03690, BSU23940, BSU18760, new_2701783_2703117, new_1253669_1254769, BSU25730 |
Expression pos. correlated with | BSU15280, BSU35490, BSU16960, BSU33040, BSU38860, BSU21830, BSU18990, BSU21890, BSU40960, BSU22880 |
Highly expressed condition | (BC) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC]. |
(BI) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC]. | |
(C90) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal. | |
(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LBtran) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(M0t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(S2) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T3.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T3.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T5.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Lowely expressed condition | (BMM) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. |
(Heat) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(LBGstat) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LPhT) Cells were harvested (i) during exponential growth in high phosphate defined medium [HPh]; (ii) during exponential growth in low phosphate defined medium [LPh] (J. P. Muller, Z. An, T. Merad, I. C. Hancock, C. R. Harwood, Microbiology 143, 947, Mar, 1997);and (iii) at three hours after the outset of the phosphate-limitation induced stationary phase [LPhT]. | |
(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T0.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Name | ftsZ |