divIVA
BSGatlas-gene-1823
BSGatlas
Description | Information |
---|---|
Coordinates | 1612521..1613015 |
Genomic Size | 495 bp |
Name | divIVA |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | + |
Type | CDS |
SubtiWiki
Description | Information |
---|---|
Alternative Name | divIVA |
divIVA | |
ylmJ | |
Category | SW 1 Cellular processes |
SW 1.1 Cell envelope and cell division | |
SW 1.1.8 Cell division | |
SW 1.1.8.1 The Min system | |
SW 1.1.8.2 Other genes | |
SW 6 Groups of genes | |
SW 6.2 Membrane proteins | |
SW 6.4 Phosphoproteins | |
SW 6.4.1 Phosphorylation on an Arg residue | |
Description | curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum, [SW|cell division] initiation protein (septum placement), part of the Min system (with Z ring placement) |
Function | division site selection, chromosome segregation and control of peptidoglycan homeostasis |
Is essential? | no |
Isoelectric point | 4.85 |
Locus Tag | BSU_15420 |
Molecular weight | 19.1967 |
Name | divIVA |
Product | [SW|cell division] initiation protein, member of the [SW|divisome] |
RefSeq
Description | Information |
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Alternative Locus Tag | BSU15420 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 10724454, 12950914,14526035, 15165232, 15554965, 16885474, 22108385,22582279, 23264578, 24129255, 28674273; Product type f :factor |
Functions | 16.13: Shape |
Locus Tag | BSU_15420 |
Name | divIVA |
Title | cell-division initiation protein |
Type | CDS |
BsubCyc
Description | Information |
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Alternative Name | ylmJ |
Citation | Bach JN;Albrecht N;Bramkamp M Imaging DivIVA dynamics using photo-convertible and activatable fluorophores in Bacillus subtilis. Front Microbiol 5;59 (2014) PUBMED: 24600441 |
Barak I Complexity of bacterial phosphorylation interaction network. Front Microbiol 5;725 (2014) PUBMED: 25566234 | |
Barak I Open questions about the function and evolution of bacterial Min systems. Front Microbiol 4;378 (2013) PUBMED: 24367361 | |
dos Santos VT;Bisson-Filho AW;Gueiros-Filho FJ DivIVA-mediated polar localization of ComN, a posttranscriptional regulator of Bacillus subtilis. J Bacteriol 194(14);3661-9 (2012) PUBMED: 22582279 | |
Eswaramoorthy P;Erb ML;Gregory JA;Silverman J;Pogliano K;Pogliano J;Ramamurthi KS Cellular architecture mediates DivIVA ultrastructure and regulates min activity in Bacillus subtilis. MBio 2(6) (2011) PUBMED: 22108385 | |
Eswaramoorthy P;Winter PW;Wawrzusin P;York AG;Shroff H;Ramamurthi KS Asymmetric Division and Differential Gene Expression during a Bacterial Developmental Program Requires DivIVA. PLoS Genet 10(8);e1004526 (2014) PUBMED: 25101664 | |
Halbedel S;Kawai M;Breitling R;Hamoen LW SecA is required for membrane targeting of the cell division protein DivIVA in vivo. Front Microbiol 5;58 (2014) PUBMED: 24592260 | |
Oliva MA;Halbedel S;Freund SM;Dutow P;Leonard TA;Veprintsev DB;Hamoen LW;Lowe J Features critical for membrane binding revealed by DivIVA crystal structure. EMBO J 29(12);1988-2001 (2010) PUBMED: 20502438 | |
Pogliano J;Pogliano N;Silverman JA Daptomycin-mediated reorganization of membrane architecture causes mislocalization of essential cell division proteins. J Bacteriol 194(17);4494-504 (2012) PUBMED: 22661688 | |
Renner LD;Eswaramoorthy P;Ramamurthi KS;Weibel DB Studying biomolecule localization by engineering bacterial cell wall curvature. PLoS One 8(12);e84143 (2013) PUBMED: 24391905 | |
Rowlett VW;Margolin W The Min system and other nucleoid-independent regulators of Z ring positioning. Front Microbiol 6;478 (2015) PUBMED: 26029202 | |
van Baarle S;Celik IN;Kaval KG;Bramkamp M;Hamoen LW;Halbedel S Protein-Protein Interaction Domains of Bacillus subtilis DivIVA. J Bacteriol 195(5);1012-21 (2013) PUBMED: 23264578 | |
Comment | 16.13: Shape |
Description | cell-division initiation protein |
Gene Ontology | GO:0000917 division septum assembly |
GO:0005515 protein binding | |
GO:0005737 cytoplasm | |
GO:0007049 cell cycle | |
GO:0030435 sporulation resulting in formation of a cellular spore | |
GO:0042802 identical protein binding | |
GO:0051301 cell division | |
Locus Tag | BSU15420 |
Molecular weight | 19.341 |
Name | divIVA |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | new_4194210_4195441_c, BSU25730, BSU17750, BSU03690, BSU35800, BSU17760, BSU17420, new_4159457_4159757_c, BSU30710, BSU31420 |
Expression pos. correlated with | BSU29610, new_1612428_1612520, BSU31400, BSU40960, BSU18800, BSU31500, BSU40970, BSU31410, BSU22720, BSU35220 |
Highly expressed condition | (BI) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC]. |
(C30) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal. | |
(C90) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal. | |
(Glucon) A 5 ml aliquot of LB medium was inoculated using frozen culture stocks. After a few hours growth at 37°C, precultures were prepared by inoculating 5 ml of M9 with this LB culture at several different dilutions usually ranging from 500- to 2000-fold. The dilution range was chosen so that one of these precultures had grown to and OD600 of 0.5 - 1.0 after overnight inculation. The chosen M9 medium precultures [at OD600 of 0.5 - 1.0] were used to inoculate 100 mL of M9 medium in 500 mL non-baffled shake flasks to an OD600 of 0.02. Filter-sterilized carbon sources were added separately to the medium M9 at following concentration: D-Glucose 3g/L[Glu], L-Malic acid 4.5g/L[Mal], L-Malic acid + D-Glucose 3 and 2g/L[M+G], D-Fructose 3g/L[Fru], D-Gluconate 4g/L[Glucon], Pyruvate 6g/L[Pyr], Glycerol 6g/L[Gly], Glutamic acid + Succinic acid 2 and 2g/L[G+S]. Where necessary, carbon source solutions were pH neutralized with 4 M NaOH prior to addition to the medium. Cells were harvested during the exponential growth phase. | |
(HiTm) Cells were grown in Spizizen’s minimal medium (SMM) (C. Anagnostopoulos, J. Spizizen, J Bacteriol 81, 741, May, 1961) with vigorous agitation. The control culture was grown at 37 °C [SMMPr]. For growth at high or low temperatures, pre-cultures were grown at 37 °C, diluted to an OD578nm of 0.1 and subsequently transferred to 51 °C [HiTm] and 16 °C [LoTm], respectively. For the growth at high salinity, the salinity of the medium was adjusted by adding NaCl (5 M stock solution) to produce a final concentration of 1.2 M [HiOs]. | |
(LBGstat) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LBGtran) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(M0t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(MG+5) A culture of LB medium was inocualted from a frozen glycerol stock of B. subtilis. After few hours at 37oC when the culture was growing exponentially, this culture was used to inoculate M9 minimal medium at several different dilutions usually in the range of 500- to 2000-fold. The dilution range was chosen to ensure that at least one of these M9 precultures had reached an OD600 between 0.5 - 1.0 after overnight incubation. These precultures were then used to inoculate 2.5 L of M9 medium in a 3.1 L KLF bioreactor (Bioengineering AG, Wald, Switzerland) to a starting OD600 of 0.03 – 0.05. Condiions in the bioreactor were rigorously controlled as follows: temperature was controlled at 37 °C; the pH was maintained at exactly 7.2 by automatic titration with 2.0 M KOH and 2.0 M H2SO4, and the dissolved oxygen tension was maintained above 50%. In each nutritional shift experiment cells were grown on the single substrate until the OD600 reached 0.50, at which point the second substrate was added instantaneously (4 g/L L-malate or 3 g/L glucose). The nutrient shifts performed were from glucose to glucose+malate [GM] and from malate to malate+glucose [MG] (Buescher et al., accompanying paper). Cell growth during the course was monitored throughout the experiment by measuring OD600. | |
Lowely expressed condition | (BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(dia15) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15]. | |
(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(LoTm) Cells were grown in Spizizen’s minimal medium (SMM) (C. Anagnostopoulos, J. Spizizen, J Bacteriol 81, 741, May, 1961) with vigorous agitation. The control culture was grown at 37 °C [SMMPr]. For growth at high or low temperatures, pre-cultures were grown at 37 °C, diluted to an OD578nm of 0.1 and subsequently transferred to 51 °C [HiTm] and 16 °C [LoTm], respectively. For the growth at high salinity, the salinity of the medium was adjusted by adding NaCl (5 M stock solution) to produce a final concentration of 1.2 M [HiOs]. | |
(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T5.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Name | divIVA |