prkC
BSGatlas-gene-1864
BSGatlas
Description | Information |
---|---|
Coordinates | 1651142..1653088 |
Genomic Size | 1947 bp |
Name | prkC |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | + |
Type | CDS |
SubtiWiki
Description | Information |
---|---|
Alternative Name | prkC |
prkC | |
yloP | |
Category | SW 3 Information processing |
SW 3.3 Protein synthesis, modification and degradation | |
SW 3.3.4 Protein modification | |
SW 3.3.4.2 Protein kinases | |
SW 4 Lifestyles | |
SW 4.2 Sporulation | |
SW 4.2.4 Germination | |
SW 4.2.4.2 Additional germination proteins | |
SW 6 Groups of genes | |
SW 6.2 Membrane proteins | |
SW 6.4 Phosphoproteins | |
SW 6.4.6 Phosphorylation on a Thr residue | |
Description | protein kinase C, induces [[category|SW 4.2.4]] of spores in response to DAP-type, and not to Lys-type cell wall muropeptides, stimulates [[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D]] activity |
Enzyme Classifications | EC 2.7.11.1: non-specific serine/threonine protein kinase |
Function | germination in response to muropeptides |
Is essential? | no |
Isoelectric point | 4.83 |
Locus Tag | BSU_15770 |
Molecular weight | 71.6872 |
Name | prkC |
Product | protein kinase |
RefSeq
Description | Information |
---|---|
Alternative Locus Tag | BSU15770 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 10986276, 12399479,18984160, 24390483, 25845974, 26102633; Product type e :enzyme |
Enzyme Classifications | EC 2.7.11.1: non-specific serine/threonine protein kinase |
Functions | 16.12: Sense |
16.3: Control | |
Locus Tag | BSU_15770 |
Name | prkC |
Title | protein serine/threonine kinase |
Type | CDS |
BsubCyc
Description | Information |
---|---|
Alternative Name | yloP |
Citation | Berisio R;Squeglia F;Ruggiero A;Petraccone L;Stellato MI;Del Vecchio P Differential thermodynamic behaviours of the extra-cellular regions of two Ser/Thr PrkC kinases revealed by calorimetric studies. Biochim Biophys Acta 1854(5);402-9 (2015) PUBMED: 25668224 |
Chen Y;Ray WK;Helm RF;Melville SB;Popham DL Levels of Germination Proteins in Bacillus subtilis Dormant, Superdormant, and Germinating Spores. PLoS One 9(4);e95781 (2014) PUBMED: 24752279 | |
Foulquier E;Pompeo F;Freton C;Cordier B;Grangeasse C;Galinier A PrkC-mediated Phosphorylation of Overexpressed YvcK Protein Regulates PBP1 Protein Localization in Bacillus subtilis mreB Mutant Cells. J Biol Chem 289(34);23662-9 (2014) PUBMED: 25012659 | |
Kobir A;Poncet S;Bidnenko V;Delumeau O;Jers C;Zouhir S;Grenha R;Nessler S;Noirot P;Mijakovic I Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties. Mol Microbiol 92(5);1129-41 (2014) PUBMED: 24731262 | |
Leiba J;Hartmann T;Cluzel ME;Cohen-Gonsaud M;Delolme F;Bischoff M;Molle V A novel mode of regulation of the Staphylococcus aureus catabolite control protein A (CcpA) mediated by Stk1 protein phosphorylation. J Biol Chem 287(52);43607-19 (2012) PUBMED: 23132867 | |
Libby EA;Goss LA;Dworkin J The Eukaryotic-Like Ser/Thr Kinase PrkC Regulates the Essential WalRK Two-Component System in Bacillus subtilis. PLoS Genet 11(6);e1005275 (2015) PUBMED: 26102633 | |
Pietack N;Becher D;Schmidl SR;Saier MH;Hecker M;Commichau FM;Stulke J In vitro Phosphorylation of Key Metabolic Enzymes from Bacillus subtilis: PrkC Phosphorylates Enzymes from Different Branches of Basic Metabolism. J Mol Microbiol Biotechnol 18(3);129-140 (2010) PUBMED: 20389117 | |
Pompeo F;Foulquier E;Serrano B;Grangeasse C;Galinier A Phosphorylation of the cell division protein GpsB regulates PrkC kinase activity through a negative feedback loop in Bacillus subtilis. Mol Microbiol 97(1);139-50 (2015) PUBMED: 25845974 | |
Ravikumar V;Shi L;Krug K;Derouiche A;Jers C;Cousin C;Kobir A;Mijakovic I;Macek B Quantitative phosphoproteome analysis of bacillus subtilis reveals novel substrates of the kinase PrkC and phosphatase PrpC. Mol Cell Proteomics 13(8);1965-78 (2014) PUBMED: 24390483 | |
Shi L;Pigeonneau N;Ravikumar V;Dobrinic P;Macek B;Franjevic D;Noirot-Gros MF;Mijakovic I Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Front Microbiol 5;495 (2014) PUBMED: 25278935 | |
Squeglia F;Marchetti R;Ruggiero A;Lanzetta R;Marasco D;Dworkin J;Petoukhov M;Molinaro A;Berisio R;Silipo A Chemical basis of peptidoglycan discrimination by PrkC, a key kinase involved in bacterial resuscitation from dormancy. J Am Chem Soc 133(51);20676-9 (2011) PUBMED: 22111897 | |
Comment | Review: |CITS: [27148245]| 16.3: Control 16.12: Sense |
Description | protein kinase |
Enzyme Classifications | EC 2.7.11.1: non-specific serine/threonine protein kinase |
EC 2.7.11.12: cGMP-dependent protein kinase | |
EC 2.7.11.13: protein kinase C | |
EC 2.7.11.17: Ca2+/calmodulin-dependent protein kinase | |
EC 2.7.11.21: polo kinase | |
EC 2.7.11.22: cyclin-dependent kinase | |
Gene Ontology | GO:0000166 nucleotide binding |
GO:0004672 protein kinase activity | |
GO:0004674 protein serine/threonine kinase activity | |
GO:0005524 ATP binding | |
GO:0005886 plasma membrane | |
GO:0006468 protein phosphorylation | |
GO:0007165 signal transduction | |
GO:0008658 penicillin binding | |
GO:0009847 spore germination | |
GO:0016020 membrane | |
GO:0016021 integral component of membrane | |
GO:0016301 kinase activity | |
GO:0016310 phosphorylation | |
GO:0016740 transferase activity | |
GO:0016772 transferase activity, transferring phosphorus-containing groups | |
GO:0042834 peptidoglycan binding | |
GO:0071224 cellular response to peptidoglycan | |
Locus Tag | BSU15770 |
Molecular weight | 71.866 |
Name | prkC |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | BSU07735, new_3105800_3106064_c, BSU30330, BSU30659, BSU40280, BSU14660, new_1537029_1537112, BSU19259, new_2098330_2098837, BSU10410 |
Expression pos. correlated with | BSU15760, BSU24140, BSU18100, BSU01770, BSU29670, BSU27700, BSU06680, BSU18090, BSU15010, BSU15020, BSU06690 |
Highly expressed condition | (G135) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. |
(G150) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(G180) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(H2O2) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(LBGexp) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LBGtran) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(Oxctl) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(Paraq) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(S0) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
Lowely expressed condition | (B36) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(B60) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. | |
(Cold) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(LPhT) Cells were harvested (i) during exponential growth in high phosphate defined medium [HPh]; (ii) during exponential growth in low phosphate defined medium [LPh] (J. P. Muller, Z. An, T. Merad, I. C. Hancock, C. R. Harwood, Microbiology 143, 947, Mar, 1997);and (iii) at three hours after the outset of the phosphate-limitation induced stationary phase [LPhT]. | |
(M9stat) Cells were grown in M9 supplemented with glucose (0.3 %) at 37°C with vigorous shaking. The composition of the M9 minimal medium is (per liter): 8.5 g Na2HPO4.2H20, 3 g KH2PO4, 1 g NH4Cl and 0.5 g NaCl. The following solutions were individually sterilized and added (volumes per liter of medium): 1 ml 0.1 M CaCl2.2H2O, 1 ml 1 M MgSO4.7H2O, 1 ml 50 mM Fe-Citrate. Also added was 10 ml of a trace salts solution containing (per liter): 170 mg ZnCl2, 100 mg MnCl2.4H2O, 60 mg CoCl2.6H2O, 60 mg Na2MoO4.2H2O and 43 mg CuCl2.2H2O. Overnight cultures were diluted 2000-fold in pre-warmed M9 medium and samples were harvested during exponential growth [M9exp], at the transition phase [M9tran] and during stationary phase [M9stat]. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T0.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Name | prkC |