polC
BSGatlas-gene-1948
BSGatlas
Description | Information |
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Coordinates | 1727133..1731446 |
Genomic Size | 4314 bp |
Name | polC |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | + |
Type | CDS |
SubtiWiki
Description | Information |
---|---|
Alternative Name | dnaF |
dnaP | |
mutI | |
polC | |
polC | |
Category | SW 3 Information processing |
SW 3.1 Genetics | |
SW 3.1.1 DNA replication | |
SW 6 Groups of genes | |
SW 6.1 Essential genes | |
Description | DNA polymerase III, leading strand polymerase (alpha subunit), part of the [SW|replisome] |
Enzyme Classifications | EC 2.7.7.7: DNA-directed DNA polymerase |
Function | [[category|SW 3.1.1]] |
Is essential? | yes |
Isoelectric point | 5.14 |
Locus Tag | BSU_16580 |
Molecular weight | 162.424 |
Name | polC |
Product | DNA polymerase III (alpha subunit) |
RefSeq
Description | Information |
---|---|
Alternative Locus Tag | BSU16580 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 11721055, 12081953,12217498, 20122408, 21740522, 24106089, 28575448; Producttype e: enzyme |
Enzyme Classifications | EC 2.7.7.7: DNA-directed DNA polymerase |
Functions | 16.9: Replicate |
Locus Tag | BSU_16580 |
Name | polC |
Title | DNA polymerase III (alpha subunit) |
Type | CDS |
BsubCyc
Description | Information |
---|---|
Alternative Name | dnaF |
dnaP | |
mutI | |
Citation | Liao Y;Li Y;Schroeder JW;Simmons LA;Biteen JS Single-Molecule DNA Polymerase Dynamics at a Bacterial Replisome in Live Cells. Biophys J 111(12);2562-2569 (2016) PUBMED: 28002733 |
Mangiameli SM;Merrikh CN;Wiggins PA;Merrikh H Transcription leads to pervasive replisome instability in bacteria. Elife 6 (2017) PUBMED: 28092263 | |
Sanders GM;Dallmann HG;McHenry CS Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases. Mol Cell 37(2);273-81 (2010) PUBMED: 20122408 | |
Schroeder JW;Hirst WG;Szewczyk GA;Simmons LA The Effect of Local Sequence Context on Mutational Bias of Genes Encoded on the Leading and Lagging Strands. Curr Biol 26(5);692-7 (2016) PUBMED: 26923786 | |
Timinskas K;Balvociutė M;Timinskas A;Venclovas Č Comprehensive analysis of DNA polymerase III α subunits and their homologs in bacterial genomes. Nucleic Acids Res 42(3);1393-413 (2014) PUBMED: 24106089 | |
Timinskas K;Venclovas Č The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III τ subunit. FEBS J 278(17);3109-18 (2011) PUBMED: 21740522 | |
Comment | Evidence 1a: Function experimentally demonstrated in the studied strain; PubMedId: 11721055, 12081953, 12217498; Product type e: enzyme |
Description | DNA polymerase III (alpha subunit) |
Enzyme Classifications | EC 2.7.7.7: DNA-directed DNA polymerase |
Gene Ontology | GO:0003676 nucleic acid binding |
GO:0003677 DNA binding | |
GO:0003824 catalytic activity | |
GO:0003887 DNA-directed DNA polymerase activity | |
GO:0004518 nuclease activity | |
GO:0004527 exonuclease activity | |
GO:0005737 cytoplasm | |
GO:0006260 DNA replication | |
GO:0006261 DNA-dependent DNA replication | |
GO:0008408 3'-5' exonuclease activity | |
GO:0016740 transferase activity | |
GO:0016779 nucleotidyltransferase activity | |
GO:0016787 hydrolase activity | |
GO:0046677 response to antibiotic | |
GO:0090305 nucleic acid phosphodiester bond hydrolysis | |
Locus Tag | BSU16580 |
Molecular weight | 162.662 |
Name | polC |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | BSU17240, BSU32950, BSU30020, BSU05020, new_3072180_3072285, new_3072287_3072400, BSU10410, BSU13940, BSU31030, BSU09889 |
Expression pos. correlated with | BSU34810, BSU00060, BSU06350, BSU40370, BSU21710, BSU35700, BSU00070, BSU15740, BSU40390, BSU15950 |
Highly expressed condition | (BC) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC]. |
(C30) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal. | |
(G135) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(G150) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(G180) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(H2O2) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(LBGexp) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(Oxctl) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(Paraq) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(Sw) Exponentially growing cells were spotted on 1 % agar LB plates and incubated at 37°C. Swarming cells were collected after 16 hours. | |
Lowely expressed condition | (Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. |
(Pyr) A 5 ml aliquot of LB medium was inoculated using frozen culture stocks. After a few hours growth at 37°C, precultures were prepared by inoculating 5 ml of M9 with this LB culture at several different dilutions usually ranging from 500- to 2000-fold. The dilution range was chosen so that one of these precultures had grown to and OD600 of 0.5 - 1.0 after overnight inculation. The chosen M9 medium precultures [at OD600 of 0.5 - 1.0] were used to inoculate 100 mL of M9 medium in 500 mL non-baffled shake flasks to an OD600 of 0.02. Filter-sterilized carbon sources were added separately to the medium M9 at following concentration: D-Glucose 3g/L[Glu], L-Malic acid 4.5g/L[Mal], L-Malic acid + D-Glucose 3 and 2g/L[M+G], D-Fructose 3g/L[Fru], D-Gluconate 4g/L[Glucon], Pyruvate 6g/L[Pyr], Glycerol 6g/L[Gly], Glutamic acid + Succinic acid 2 and 2g/L[G+S]. Where necessary, carbon source solutions were pH neutralized with 4 M NaOH prior to addition to the medium. Cells were harvested during the exponential growth phase. | |
(S3) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T5.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Name | polC |
KEGG Pathways
Description | Information |
---|---|
Pathway | DNA replication (ko03030) |
Mismatch repair (ko03430) | |
Homologous recombination (ko03440) |