BSGatlas

glmS

BSGatlas-gene-247

BSGatlas

Description	Information
Coordinates	200277..202079
Genomic Size	1803 bp
Name	glmS
Outside Links	SubtiWiki
	BsubCyc
Strand	+
Type	CDS

SubtiWiki

Description	Information
Alternative Name	gcaA
	glmS
	glmS
	ybxD
Category	SW 1 Cellular processes
	SW 1.1 Cell envelope and cell division
	SW 1.1.1 Cell wall synthesis
	SW 1.1.1.1 Biosynthesis of peptidoglycan
	SW 2 Metabolism
	SW 2.6 Additional metabolic pathways
	SW 2.6.1 Biosynthesis of cell wall components
	SW 2.6.1.1 Biosynthesis of peptidoglycan
	SW 6 Groups of genes
	SW 6.1 Essential genes
Description	glutamine-fructose-6-phosphate transaminase
Enzyme Classifications	EC 2.6.1.16: glutamine-fructose-6-phosphate transaminase (isomerizing)
Function	cell wall synthesis
Is essential?	yes
Isoelectric point	4.8
Locus Tag	BSU_01780
Molecular weight	65.1628
Name	glmS
Product	glutamine-fructose-6-phosphate transaminase

RefSeq

Description	Information
Alternative Locus Tag	BSU01780
Description	Evidence 2a: Function from experimental evidencesin other organisms; PubMedId: 12682299, 15029187,17114942, 17283212, 17322533, 23874669; Product type e :enzyme
Enzyme Classifications	EC 2.6.1.16: glutamine-fructose-6-phosphate transaminase (isomerizing)
Functions	16.13: Shape
	16.2: Construct biomass (Anabolism)
Locus Tag	BSU_01780
Name	glmS
Title	L-glutamine-D-fructose-6-phosphateamidotransferase
Type	CDS

BsubCyc

Description	Information
Alternative Name	gcaA
	ybxD
Citation	Blount K;Puskarz I;Penchovsky R;Breaker R Development and application of a high-throughput assay for glmS riboswitch activators. RNA Biol 3(2);77-81 (2006) PUBMED: 17114942
	Brooks KM;Hampel KJ Rapid steps in the glmS ribozyme catalytic pathway: cation and ligand requirements. Biochemistry 50(13);2424-33 (2011) PUBMED: 21395279
	Li Y;Zhong C;Zhang S Finding consensus stable local optimal structures for aligned RNA sequences and its application to discovering riboswitch elements. Int J Bioinform Res Appl 10(4-5);498-518 (2014) PUBMED: 24989865
	Zhao Y;Chen H;Du F;Yasmeen A;Dong J;Cui X;Tang Z Signal amplification of glucosamine-6-phosphate based on ribozyme glmS. Biosens Bioelectron 62;337-42 (2014) PUBMED: 25038539
Comment	Glucosamine--fructose-6-phosphate aminotransferase (GlmS) is the first enzyme of the \|FRAME: UDPNAGSYN-PWY\| pathway. The B. subtilis enzyme has not been purified. GlmS is essential for growth \|CITS: [12682299]\|. Expression of glmS is repressed in response to rising glucosamine-6-phosphage (GlcN6P) concentrations, which are sensed by the 5' UTR RNA of the glmS transcript. Unlike many riboswitches, binding of GlcN6P does not lead to a conformational change in the RNA \|CITS: [17283212]\|, but rather activates the GlcN6P-sensing ribozyme, leading to cleavage of the mRNA \|CITS: [15029187]\|. This metabolite-induced cleavage targets the downstream transcript for degradation by \|FRAME: BSU14530-MONOMER "RNase J1"\| \|CITS: [18079181]\|. It was recently shown that the glmS riboswitch integrates both positive and negative metabolic signals \|CITS: [21317896]\|. Review: \|CITS: [12044898]\|
Description	L-glutamine-D-fructose-6-phosphate amidotransferase
Enzyme Classifications	EC 2.6.1.16: glutamine-fructose-6-phosphate transaminase (isomerizing)
Gene Ontology	GO:0004360 glutamine-fructose-6-phosphate transaminase (isomerizing) activity
	GO:0005737 cytoplasm
	GO:0005975 carbohydrate metabolic process
	GO:0006541 glutamine metabolic process
	GO:0008152 metabolic process
	GO:0008483 transaminase activity
	GO:0016051 carbohydrate biosynthetic process
	GO:0016740 transferase activity
	GO:0030246 carbohydrate binding
Locus Tag	BSU01780
Molecular weight	65.338
Name	glmS

Nicolas et al. predictions

Description	Information
Expression neg. correlated with	BSU19230, BSU30690, new_4109864_4110916_c, new_3589361_3589438_c, new_2879205_2879294_c, BSU10090, BSU14680, new_2069746_2069982_c, new_3046584_3046684_c, new_2586704_2586996_c
Expression pos. correlated with	new_200188_200276, BSU14880, BSU09260, BSU09270, new_1534271_1535874, new_2417904_2417983_c, BSU28800, BSU28510, BSU15860, BSU28790
Highly expressed condition	(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(M0t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90].
	(M40t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90].
	(M9stat) Cells were grown in M9 supplemented with glucose (0.3 %) at 37°C with vigorous shaking. The composition of the M9 minimal medium is (per liter): 8.5 g Na2HPO4.2H20, 3 g KH2PO4, 1 g NH4Cl and 0.5 g NaCl. The following solutions were individually sterilized and added (volumes per liter of medium): 1 ml 0.1 M CaCl2.2H2O, 1 ml 1 M MgSO4.7H2O, 1 ml 50 mM Fe-Citrate. Also added was 10 ml of a trace salts solution containing (per liter): 170 mg ZnCl2, 100 mg MnCl2.4H2O, 60 mg CoCl2.6H2O, 60 mg Na2MoO4.2H2O and 43 mg CuCl2.2H2O. Overnight cultures were diluted 2000-fold in pre-warmed M9 medium and samples were harvested during exponential growth [M9exp], at the transition phase [M9tran] and during stationary phase [M9stat].
	(T1.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
	(T1.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
	(T2.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
	(T2.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
	(T3.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
	(T3.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
Lowely expressed condition	(Cold) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(dia5) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15].
	(Diami) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition
	(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(HiOs) Cells were grown in Spizizen’s minimal medium (SMM) (C. Anagnostopoulos, J. Spizizen, J Bacteriol 81, 741, May, 1961) with vigorous agitation. The control culture was grown at 37 °C [SMMPr]. For growth at high or low temperatures, pre-cultures were grown at 37 °C, diluted to an OD578nm of 0.1 and subsequently transferred to 51 °C [HiTm] and 16 °C [LoTm], respectively. For the growth at high salinity, the salinity of the medium was adjusted by adding NaCl (5 M stock solution) to produce a final concentration of 1.2 M [HiOs].
	(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(Salt) Cells were grown in Spizizen’s minimal medium (SMM) at 37 °C with vigorous shaking. Salt was added, to a final concentration of 0.4 M to an exponentially growing culture of cells at OD500 of 0.4. Samples were harvested before [SMM] and 10 minutes after [Salt] NaCl addition.
	(T-0.40H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
	(T-1.10H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
	(T0.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
Name	glmS

KEGG Pathways

Description	Information
Pathway	Alanine, aspartate and glutamate metabolism (ko00250)
	Amino sugar and nucleotide sugar metabolism (ko00520)
	Metabolic pathways (ko01100)

Fullscreen (Open in UCSC browser US | EU )