ugtP
BSGatlas-gene-2578
BSGatlas
Description | Information |
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Coordinates | 2306514..2307662 |
Genomic Size | 1149 bp |
Name | ugtP |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | + |
Type | CDS |
SubtiWiki
Description | Information |
---|---|
Alternative Name | ugtP |
ugtP | |
ypfP | |
Category | SW 1 Cellular processes |
SW 1.1 Cell envelope and cell division | |
SW 1.1.2 Cell shape | |
SW 1.1.8 Cell division | |
SW 1.1.8.2 Other genes | |
SW 2 Metabolism | |
SW 2.4 Lipid metabolism | |
SW 2.4.3 Lipid metabolism/ other | |
SW 4 Lifestyles | |
SW 4.3 Coping with stress | |
SW 4.3.2 Cell envelope stress proteins (controlled by SigM, V, W, X, Y) | |
SW 6 Groups of genes | |
SW 6.2 Membrane proteins | |
Description | UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of [[category|SW 1.1.8]] |
Function | synthesis of glucolipids and anchoring of lipoteichoic acid, inhibition of [[protein|41872E2EF00C79918DD077F2EF78F37E24FEB110]] assembly |
Is essential? | no |
Isoelectric point | 8.4 |
Locus Tag | BSU_21920 |
Molecular weight | 43.4022 |
Name | ugtP |
Product | UDP-glucose diacylglycerol glucosyltransferase |
RefSeq
Description | Information |
---|---|
Alternative Locus Tag | BSU21920 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 11344159, 17209021,17662947, 9720862, 18820022, 22362028, 22931116, 27684739;Product type e: enzyme |
Functions | 16.13: Shape |
Locus Tag | BSU_21920 |
Name | ugtP |
Title | UDP-glucose diacylglyceroltransferase |
Type | CDS |
BsubCyc
Description | Information |
---|---|
Alternative Name | ypfP |
Citation | Chien AC;Zareh SK;Wang YM;Levin PA Changes in the oligomerization potential of the division inhibitor UgtP co-ordinate Bacillus subtilis cell size with nutrient availability. Mol Microbiol 86(3);594-610 (2012) PUBMED: 22931116 |
Hill NS;Buske PJ;Shi Y;Levin PA A Moonlighting Enzyme Links Escherichia coli Cell Size with Central Metabolism. PLoS Genet 9(7);e1003663 (2013) PUBMED: 23935518 | |
Johnson CM;Grossman AD Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis. Mol Microbiol 93(6);1284-301 (2014) PUBMED: 25069588 | |
Johnson CM;Grossman AD The Composition of the Cell Envelope Affects Conjugation in Bacillus subtilis. J Bacteriol 198(8);1241-9 (2016) PUBMED: 26833415 | |
Matsuoka S;Chiba M;Tanimura Y;Hashimoto M;Hara H;Matsumoto K Abnormal morphology of Bacillus subtilis ugtP mutant cells lacking glucolipids. Genes Genet Syst 86(5);295-304 (2011) PUBMED: 22362028 | |
Matsuoka S;Seki T;Matsumoto K;Hara H Suppression of abnormal morphology and extracytoplasmic function sigma activity in Bacillus subtilis ugtP mutant cells by expression of heterologous glucolipid synthases from Acholeplasma laidlawii. Biosci Biotechnol Biochem 80(12);2325-2333 (2016) PUBMED: 27684739 | |
Seki T;Mineshima R;Hashimoto M;Matsumoto K;Hara H;Matsuoka S Repression of the activities of two extracytoplasmic function σ factors, σ(M) and σ(V), of Bacillus subtilis by glucolipids in Escherichia coli cells. Genes Genet Syst 90(2);109-14 (2015) PUBMED: 26399770 | |
Weart RB;Lee AH;Chien AC;Haeusser DP;Hill NS;Levin PA A metabolic sensor governing cell size in bacteria. Cell 130(2);335-47 (2007) PUBMED: 17662947 | |
Comment | 16.13: Shape |
Description | UDP-glucose diacylglyceroltransferase |
Gene Ontology | GO:0005515 protein binding |
GO:0005886 plasma membrane | |
GO:0005975 carbohydrate metabolic process | |
GO:0009058 biosynthetic process | |
GO:0009246 enterobacterial common antigen biosynthetic process | |
GO:0009247 glycolipid biosynthetic process | |
GO:0016020 membrane | |
GO:0016740 transferase activity | |
GO:0016757 transferase activity, transferring glycosyl groups | |
GO:0016758 transferase activity, transferring hexosyl groups | |
GO:0046527 glucosyltransferase activity | |
GO:0047228 1,2-diacylglycerol 3-glucosyltransferase activity | |
GO:0070395 lipoteichoic acid biosynthetic process | |
Locus Tag | BSU21920 |
Molecular weight | 43.562 |
Name | ugtP |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | BSU29150, BSU32360, BSU13930, BSU13920, new_3016405_3016534_c, BSU01520, BSU13040, new_1313663_1313744_c, BSU10080, BSU37840 |
Expression pos. correlated with | BSU15380, BSU15370, BSU18890, BSU00990, new_2057588_2058714_c, BSU14850, BSU22820, BSU22810, BSU20560, BSU01000 |
Highly expressed condition | (BC) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC]. |
(C30) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal. | |
(C90) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal. | |
(Heat) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(HiTm) Cells were grown in Spizizen’s minimal medium (SMM) (C. Anagnostopoulos, J. Spizizen, J Bacteriol 81, 741, May, 1961) with vigorous agitation. The control culture was grown at 37 °C [SMMPr]. For growth at high or low temperatures, pre-cultures were grown at 37 °C, diluted to an OD578nm of 0.1 and subsequently transferred to 51 °C [HiTm] and 16 °C [LoTm], respectively. For the growth at high salinity, the salinity of the medium was adjusted by adding NaCl (5 M stock solution) to produce a final concentration of 1.2 M [HiOs]. | |
(LPhT) Cells were harvested (i) during exponential growth in high phosphate defined medium [HPh]; (ii) during exponential growth in low phosphate defined medium [LPh] (J. P. Muller, Z. An, T. Merad, I. C. Hancock, C. R. Harwood, Microbiology 143, 947, Mar, 1997);and (iii) at three hours after the outset of the phosphate-limitation induced stationary phase [LPhT]. | |
(T2.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T3.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T4.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T5.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Lowely expressed condition | (B36) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. | |
(dia15) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15]. | |
(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(Gly) A 5 ml aliquot of LB medium was inoculated using frozen culture stocks. After a few hours growth at 37°C, precultures were prepared by inoculating 5 ml of M9 with this LB culture at several different dilutions usually ranging from 500- to 2000-fold. The dilution range was chosen so that one of these precultures had grown to and OD600 of 0.5 - 1.0 after overnight inculation. The chosen M9 medium precultures [at OD600 of 0.5 - 1.0] were used to inoculate 100 mL of M9 medium in 500 mL non-baffled shake flasks to an OD600 of 0.02. Filter-sterilized carbon sources were added separately to the medium M9 at following concentration: D-Glucose 3g/L[Glu], L-Malic acid 4.5g/L[Mal], L-Malic acid + D-Glucose 3 and 2g/L[M+G], D-Fructose 3g/L[Fru], D-Gluconate 4g/L[Glucon], Pyruvate 6g/L[Pyr], Glycerol 6g/L[Gly], Glutamic acid + Succinic acid 2 and 2g/L[G+S]. Where necessary, carbon source solutions were pH neutralized with 4 M NaOH prior to addition to the medium. Cells were harvested during the exponential growth phase. | |
(S0) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T0.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Name | ugtP |
KEGG Pathways
Description | Information |
---|---|
Pathway | Glycerolipid metabolism (ko00561) |
Metabolic pathways (ko01100) |