BSGatlas

spoIVA

BSGatlas-gene-2679

BSGatlas

Description	Information
Coordinates	2386195..2387673
Genomic Size	1479 bp
Name	spoIVA
Outside Links	SubtiWiki
	BsubCyc
Strand	-
Type	CDS

SubtiWiki

Description	Information
Alternative Name	spoIVA
	spoIVA
	spoVP
Category	SW 4 Lifestyles
	SW 4.2 Sporulation
	SW 4.2.1 Sporulation proteins
	SW 4.2.1.1 Spore coat proteins
	SW 4.2.1.1.1 Class I
	SW 6 Groups of genes
	SW 6.4 Phosphoproteins
	SW 6.4.1 Phosphorylation on an Arg residue
Description	ATPase, spore coat morphogenetic protein, anchors the spore coat to the spore surface via [[protein\|BBDA32A4EE3389D6F4404F7B3DA9E13AC7BF8055]]
Function	initiation of spore coat assembly
Is essential?	no
Isoelectric point	4.55
Locus Tag	BSU_22800
Molecular weight	55.0071
Name	spoIVA
Product	ATPase, basement layer protein for spore coat assembly

RefSeq

Description	Information
Alternative Locus Tag	BSU22800
Description	Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 11160095, 17114257,17427285, 24810258, 26387458, 28408070; Product type cp :cell process
Functions	16.5: Explore
Locus Tag	BSU_22800
Name	spoIVA
Title	morphogenetic stage IV sporulation protein
Type	CDS

BsubCyc

Description	Information
Alternative Name	spoVP
Citation	Castaing JP;Lee S;Anantharaman V;Ravilious GE;Aravind L;Ramamurthi KS An autoinhibitory conformation of the Bacillus subtilis spore coat protein SpoIVA prevents its premature ATP-independent aggregation. FEMS Microbiol Lett 358(2);145-53 (2014) PUBMED: 24810258
	Castaing JP;Nagy A;Anantharaman V;Aravind L;Ramamurthi KS ATP hydrolysis by a domain related to translation factor GTPases drives polymerization of a static bacterial morphogenetic protein. Proc Natl Acad Sci U S A 110(2);E151-60 (2013) PUBMED: 23267091
	Meneghini MD Spore No More: Quality Control during Bacterial Development. Dev Cell 34(6);611-2 (2015) PUBMED: 26418292
	Qiao H;Krajcikova D;Liu C;Li Y;Wang H;Barak I;Tang J The interactions of spore-coat morphogenetic proteins studied by single-molecule recognition force spectroscopy. Chem Asian J 7(4);725-31 (2012) PUBMED: 22262582
	Tan IS;Weiss CA;Popham DL;Ramamurthi KS A Quality-Control Mechanism Removes Unfit Cells from a Population of Sporulating Bacteria. Dev Cell 34(6);682-93 (2015) PUBMED: 26387458
	Wang KH;Isidro AL;Domingues L;Eskandarian HA;McKenney PT;Drew K;Grabowski P;Chua MH;Barry SN;Guan M;Bonneau R;Henriques AO;Eichenberger P The coat morphogenetic protein SpoVID is necessary for spore encasement in Bacillus subtilis. Mol Microbiol 74(3);634-49 (2009) PUBMED: 19775244
Comment	16.5: Explore
Description	morphogenetic stage IV sporulation protein
Gene Ontology	GO:0000166 nucleotide binding
	GO:0000270 peptidoglycan metabolic process
	GO:0005515 protein binding
	GO:0005524 ATP binding
	GO:0005737 cytoplasm
	GO:0009847 spore germination
	GO:0016787 hydrolase activity
	GO:0016887 ATPase activity
	GO:0030435 sporulation resulting in formation of a cellular spore
	GO:0031160 spore wall
	GO:0042244 spore wall assembly
	GO:0042601 endospore-forming forespore
	GO:0043595 endospore cortex
	GO:0043934 sporulation
	GO:0051258 protein polymerization
	GO:0051259 protein complex oligomerization
	GO:0070590 spore wall biogenesis
Locus Tag	BSU22800
Molecular weight	55.175
Name	spoIVA

Nicolas et al. predictions

Description	Information
Expression neg. correlated with	BSU10640, new_1143506_1143576, BSU33210, BSU27370, BSU02440, BSU10650, BSU05090, BSU00580, BSU33221, BSU02580
Expression pos. correlated with	BSU08970, BSU06910, BSU06900, new_2387674_2387835_c, BSU36420, new_756987_757065, BSU36430, new_3748336_3748420_c, BSU19270, BSU14250
Highly expressed condition	(B36) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60].
	(BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60].
	(LoTm) Cells were grown in Spizizen’s minimal medium (SMM) (C. Anagnostopoulos, J. Spizizen, J Bacteriol 81, 741, May, 1961) with vigorous agitation. The control culture was grown at 37 °C [SMMPr]. For growth at high or low temperatures, pre-cultures were grown at 37 °C, diluted to an OD578nm of 0.1 and subsequently transferred to 51 °C [HiTm] and 16 °C [LoTm], respectively. For the growth at high salinity, the salinity of the medium was adjusted by adding NaCl (5 M stock solution) to produce a final concentration of 1.2 M [HiOs].
	(S3) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(T5.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
Lowely expressed condition	(C30) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal.
	(dia0) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15].
	(dia15) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15].
	(dia5) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15].
	(Diami) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition
	(HPh) Cells were harvested (i) during exponential growth in high phosphate defined medium [HPh]; (ii) during exponential growth in low phosphate defined medium [LPh] (J. P. Muller, Z. An, T. Merad, I. C. Hancock, C. R. Harwood, Microbiology 143, 947, Mar, 1997);and (iii) at three hours after the outset of the phosphate-limitation induced stationary phase [LPhT].
	(LBexp) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(LBtran) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(M40t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90].
	(S0) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
Name	spoIVA

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