luxS
BSGatlas-gene-3581
BSGatlas
Description | Information |
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Coordinates | 3137495..3137968 |
Genomic Size | 474 bp |
Name | luxS |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | - |
Type | CDS |
SubtiWiki
Description | Information |
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Alternative Name | luxS |
luxS | |
ytjB | |
Category | SW 2 Metabolism |
SW 2.3 Amino acid/ nitrogen metabolism | |
SW 2.3.1 Biosynthesis/ acquisition of amino acids | |
SW 2.3.1.10 Biosynthesis/ acquisition of methionine/ S-adenosylmethionine | |
SW 3 Information processing | |
SW 3.4 Regulation of gene expression | |
SW 3.4.8 Quorum sensing | |
SW 4 Lifestyles | |
SW 4.1 Exponential and early post-exponential lifestyles | |
SW 4.1.1 Motility and chemotaxis | |
SW 4.1.1.1 Signal transduction in motility and chemotaxis | |
SW 4.1.1.1.5 Additional chemotaxis signal transduction and regulatory proteins | |
SW 4.1.2 Biofilm formation | |
SW 4.1.2.5 Other proteins required for biofilm formation | |
Description | S-ribosylhomocysteine lyase, autoinducer-2 production protein, required for surface spreading motility (sliding) of B. subtilis natto and biofilm formation |
Enzyme Classifications | EC 4.4.1.21: S-ribosylhomocysteine lyase |
Function | methionine salvage |
Is essential? | no |
Isoelectric point | 5.17 |
Locus Tag | BSU_30670 |
Molecular weight | 17.5714 |
Name | luxS |
Product | S-ribosylhomocysteine lyase |
RefSeq
Description | Information |
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Alternative Locus Tag | BSU30670 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 11601850, 15287744,15751951, 16740951, 17056751, 22720735, 22938038,26779171, 28342077; Product type e: enzyme |
Enzyme Classifications | EC 4.4.1.21: S-ribosylhomocysteine lyase |
Functions | 16.12: Sense |
16.3: Control | |
16.5: Explore | |
Locus Tag | BSU_30670 |
Name | luxS |
Title | S-ribosylhomocysteine lyase |
Type | CDS |
BsubCyc
Description | Information |
---|---|
Alternative Name | ytjB |
Citation | Chi BK;Roberts AA;Huyen TT;Basell K;Becher D;Albrecht D;Hamilton CJ;Antelmann H S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria. Antioxid Redox Signal 18(11);1273-95 (2013) PUBMED: 22938038 |
Duanis-Assaf D;Steinberg D;Chai Y;Shemesh M The LuxS Based Quorum Sensing Governs Lactose Induced Biofilm Formation by Bacillus subtilis. Front Microbiol 6;1517 (2015) PUBMED: 26779171 | |
Malladi VL;Sobczak AJ;Meyer TM;Pei D;Wnuk SF Inhibition of LuxS by S-ribosylhomocysteine analogues containing a [4-aza]ribose ring. Bioorg Med Chem 19(18);5507-19 (2011) PUBMED: 21855358 | |
Wang D;Lin Z;Huo Z;Wang T;Yao Z;Cong Y Mechanism-based QSAR Models for the Toxicity of Quorum Sensing Inhibitors to Gram-negative and Gram-positive Bacteria. Bull Environ Contam Toxicol 97(1);145-50 (2016) PUBMED: 27084097 | |
Comment | One or more strains containing mutant alleles of this gene can be obtained from the Bacillus Genetic Stock Center. Click here to see the list of available strains at BGSC. Evidence 1a: Function experimentally demonstrated in the studied strain; PubMedId: 11601850, 15287744, 15751951, 16740951, 17056751; Product type e: enzyme A ΔluxS::cat mutant does not produce AI-2 and grows poorly with methionine as the sole sulfur source |CITS: [17056751]|. |
Description | S-ribosylhomocysteine lyase |
Enzyme Classifications | EC 4.4.1.21: S-ribosylhomocysteine lyase |
Gene Ontology | GO:0003824 catalytic activity |
GO:0005506 iron ion binding | |
GO:0008152 metabolic process | |
GO:0009087 methionine catabolic process | |
GO:0009372 quorum sensing | |
GO:0016829 lyase activity | |
GO:0043768 S-ribosylhomocysteine lyase activity | |
GO:0046872 metal ion binding | |
Locus Tag | BSU30670 |
Molecular weight | 17.714 |
Name | luxS |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | BSU01780, BSU30760, new_200188_200276, BSU30750, BSU30740, new_3923478_3923862, new_3983174_3984118, BSU30770, BSU25280, BSU25289 |
Expression pos. correlated with | new_3137969_3138320_c, BSU30690, BSU00730, BSU29380, BSU29390, BSU29370, new_965798_965868, BSU29350, BSU03180, BSU29360 |
Highly expressed condition | (B36) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(B60) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. | |
(BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. | |
(C90) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal. | |
(dia15) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15]. | |
(dia5) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15]. | |
(Diami) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(S1) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
Lowely expressed condition | (LBGstat) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . |
(S0) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T-0.40H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T-1.40H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T0.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T0.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T1.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
(T1.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Name | luxS |
KEGG Pathways
Description | Information |
---|---|
Pathway | Cysteine and methionine metabolism (ko00270) |
Metabolic pathways (ko01100) | |
Biosynthesis of amino acids (ko01230) | |
Quorum sensing (ko02024) |