comA
BSGatlas-gene-3720
BSGatlas
Description | Information |
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Coordinates | 3252804..3253448 |
Genomic Size | 645 bp |
Name | comA |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | - |
Type | CDS |
SubtiWiki
Description | Information |
---|---|
Alternative Name | comA |
comA | |
comAA | |
srfB | |
Category | SW 3 Information processing |
SW 3.1 Genetics | |
SW 3.1.7 Genetic competence | |
SW 3.4 Regulation of gene expression | |
SW 3.4.2 Transcription factors and their control | |
SW 3.4.2.1 Two-component system response regulators | |
SW 3.4.8 Quorum sensing | |
SW 4 Lifestyles | |
SW 4.1 Exponential and early post-exponential lifestyles | |
SW 4.1.3 Genetic competence | |
SW 6 Groups of genes | |
SW 6.4 Phosphoproteins | |
SW 6.4.2 Phosphorylation on an Asp residue | |
Description | two-component system response regulator, controls gene expression in response to cell density (concentration of [[protein|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E]]) |
Enzyme Classifications | EC 4.4.1.19: phosphosulfolactate synthase |
Function | regulation of genetic competence and quorum sensing |
Is essential? | no |
Isoelectric point | 4.76 |
Locus Tag | BSU_31680 |
Molecular weight | 23.9815 |
Name | comA |
Product | two-component system response regulator |
RefSeq
Description | Information |
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Alternative Locus Tag | BSU31680 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 10972833, 11179649,11717295, 12950917, 16816200, 17827323, 26582911; Producttype r: regulator |
Functions | 16.3: Control |
Locus Tag | BSU_31680 |
Name | comA |
Title | two-component response quorum-sensing regulator |
Type | CDS |
BsubCyc
Description | Information |
---|---|
Alternative Name | comAA |
srfB | |
Citation | Baker MD;Neiditch MB Structural basis of response regulator inhibition by a bacterial anti-activator protein. PLoS Biol 9(12);e1001226 (2011) PUBMED: 22215984 |
Boguslawski KM;Hill PA;Griffith KL Novel mechanisms of controlling the activities of the transcription factors Spo0A and ComA by the plasmid-encoded quorum sensing regulators Rap60-Phr60 in Bacillus subtilis. Mol Microbiol 96(2);325-48 (2015) PUBMED: 25598361 | |
Dogsa I;Choudhary KS;Marsetic Z;Hudaiberdiev S;Vera R;Pongor S;Mandic-Mulec I ComQXPA Quorum Sensing Systems May Not Be Unique to Bacillus subtilis: A Census in Prokaryotic Genomes. PLoS One 9(5);e96122 (2014) PUBMED: 24788106 | |
Hobbs CA;Bobay BG;Thompson RJ;Perego M;Cavanagh J NMR solution structure and DNA-binding model of the DNA-binding domain of competence protein A. J Mol Biol 398(2);248-63 (2010) PUBMED: 20302877 | |
Oslizlo A;Stefanic P;Dogsa I;Mandic-Mulec I Private link between signal and response in Bacillus subtilis quorum sensing. Proc Natl Acad Sci U S A 111(4);1586-91 (2014) PUBMED: 24425772 | |
Pollak S;Omer-Bendori S;Even-Tov E;Lipsman V;Bareia T;Ben-Zion I;Eldar A Facultative cheating supports the coexistence of diverse quorum-sensing alleles. Proc Natl Acad Sci U S A 113(8);2152-7 (2016) PUBMED: 26787913 | |
Wolf D;Rippa V;Mobarec JC;Sauer P;Adlung L;Kolb P;Bischofs IB The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. Nucleic Acids Res (2015) PUBMED: 26582911 | |
Zafra O;Lamprecht-Grandio M;de Figueras CG;Gonzalez-Pastor JE Extracellular DNA Release by Undomesticated Bacillus subtilis Is Regulated by Early Competence. PLoS One 7(11);e48716 (2012) PUBMED: 23133654 | |
Comment | 16.3: Control |
Description | ComA two-component response regulator, phosphorylated;ComA two-component response regulator |
Enzyme Classifications | EC 4.4.1.19: phosphosulfolactate synthase |
Gene Ontology | GO:0000156 phosphorelay response regulator activity |
GO:0000160 phosphorelay signal transduction system | |
GO:0003677 DNA binding | |
GO:0003700 DNA-binding transcription factor activity | |
GO:0005737 cytoplasm | |
GO:0005829 cytosol | |
GO:0006351 transcription, DNA-templated | |
GO:0006355 regulation of transcription, DNA-templated | |
GO:0030420 establishment of competence for transformation | |
GO:0035556 intracellular signal transduction | |
Locus Tag | BSU31680 |
Molecular weight | 24.128 |
Name | comA |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | new_930671_930735, new_1445542_1445637, BSU13790, BSU13789, new_3217848_3218524, BSU30640, BSU13799, new_3574321_3577707, BSU36790, new_2562914_2563888 |
Expression pos. correlated with | BSU31670, BSU22810, BSU22820, BSU15830, BSU19050, BSU33040, BSU36870, BSU19060, BSU17940, BSU36860 |
Highly expressed condition | (Glucon) A 5 ml aliquot of LB medium was inoculated using frozen culture stocks. After a few hours growth at 37°C, precultures were prepared by inoculating 5 ml of M9 with this LB culture at several different dilutions usually ranging from 500- to 2000-fold. The dilution range was chosen so that one of these precultures had grown to and OD600 of 0.5 - 1.0 after overnight inculation. The chosen M9 medium precultures [at OD600 of 0.5 - 1.0] were used to inoculate 100 mL of M9 medium in 500 mL non-baffled shake flasks to an OD600 of 0.02. Filter-sterilized carbon sources were added separately to the medium M9 at following concentration: D-Glucose 3g/L[Glu], L-Malic acid 4.5g/L[Mal], L-Malic acid + D-Glucose 3 and 2g/L[M+G], D-Fructose 3g/L[Fru], D-Gluconate 4g/L[Glucon], Pyruvate 6g/L[Pyr], Glycerol 6g/L[Gly], Glutamic acid + Succinic acid 2 and 2g/L[G+S]. Where necessary, carbon source solutions were pH neutralized with 4 M NaOH prior to addition to the medium. Cells were harvested during the exponential growth phase. |
(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(M0t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(M40t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(MG-0.1) A culture of LB medium was inocualted from a frozen glycerol stock of B. subtilis. After few hours at 37oC when the culture was growing exponentially, this culture was used to inoculate M9 minimal medium at several different dilutions usually in the range of 500- to 2000-fold. The dilution range was chosen to ensure that at least one of these M9 precultures had reached an OD600 between 0.5 - 1.0 after overnight incubation. These precultures were then used to inoculate 2.5 L of M9 medium in a 3.1 L KLF bioreactor (Bioengineering AG, Wald, Switzerland) to a starting OD600 of 0.03 – 0.05. Condiions in the bioreactor were rigorously controlled as follows: temperature was controlled at 37 °C; the pH was maintained at exactly 7.2 by automatic titration with 2.0 M KOH and 2.0 M H2SO4, and the dissolved oxygen tension was maintained above 50%. In each nutritional shift experiment cells were grown on the single substrate until the OD600 reached 0.50, at which point the second substrate was added instantaneously (4 g/L L-malate or 3 g/L glucose). The nutrient shifts performed were from glucose to glucose+malate [GM] and from malate to malate+glucose [MG] (Buescher et al., accompanying paper). Cell growth during the course was monitored throughout the experiment by measuring OD600. | |
(MG-0.2) A culture of LB medium was inocualted from a frozen glycerol stock of B. subtilis. After few hours at 37oC when the culture was growing exponentially, this culture was used to inoculate M9 minimal medium at several different dilutions usually in the range of 500- to 2000-fold. The dilution range was chosen to ensure that at least one of these M9 precultures had reached an OD600 between 0.5 - 1.0 after overnight incubation. These precultures were then used to inoculate 2.5 L of M9 medium in a 3.1 L KLF bioreactor (Bioengineering AG, Wald, Switzerland) to a starting OD600 of 0.03 – 0.05. Condiions in the bioreactor were rigorously controlled as follows: temperature was controlled at 37 °C; the pH was maintained at exactly 7.2 by automatic titration with 2.0 M KOH and 2.0 M H2SO4, and the dissolved oxygen tension was maintained above 50%. In each nutritional shift experiment cells were grown on the single substrate until the OD600 reached 0.50, at which point the second substrate was added instantaneously (4 g/L L-malate or 3 g/L glucose). The nutrient shifts performed were from glucose to glucose+malate [GM] and from malate to malate+glucose [MG] (Buescher et al., accompanying paper). Cell growth during the course was monitored throughout the experiment by measuring OD600. | |
(S1) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S2) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S3) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
Lowely expressed condition | (B36) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(B60) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. | |
(BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. | |
(Diami) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(LBGstat) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(LPhT) Cells were harvested (i) during exponential growth in high phosphate defined medium [HPh]; (ii) during exponential growth in low phosphate defined medium [LPh] (J. P. Muller, Z. An, T. Merad, I. C. Hancock, C. R. Harwood, Microbiology 143, 947, Mar, 1997);and (iii) at three hours after the outset of the phosphate-limitation induced stationary phase [LPhT]. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(Salt) Cells were grown in Spizizen’s minimal medium (SMM) at 37 °C with vigorous shaking. Salt was added, to a final concentration of 0.4 M to an exponentially growing culture of cells at OD500 of 0.4. Samples were harvested before [SMM] and 10 minutes after [Salt] NaCl addition. | |
(Sw) Exponentially growing cells were spotted on 1 % agar LB plates and incubated at 37°C. Swarming cells were collected after 16 hours. | |
Name | comA |
KEGG Pathways
Description | Information |
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Pathway | Two-component system (ko02020) |
Quorum sensing (ko02024) |