BSGatlas

rsbP

BSGatlas-gene-3992

BSGatlas

Description	Information
Coordinates	3500386..3501597
Genomic Size	1212 bp
Name	rsbP
Outside Links	SubtiWiki
	BsubCyc
Strand	+
Type	CDS

SubtiWiki

Description	Information
Alternative Name	rsbP
	rsbP
	yvfP
Category	SW 3 Information processing
	SW 3.3 Protein synthesis, modification and degradation
	SW 3.3.4 Protein modification
	SW 3.3.4.4 Protein phosphatases
	SW 3.4 Regulation of gene expression
	SW 3.4.1 Sigma factors and their control
	SW 3.4.1.2 Control of sigma factors
Description	protein serine phosphatase, energy PP2C, dephosphorylates [[protein\|AC64DA463250A090A62E50901EFE653C8F963872]], required for activation of [[protein\|580011DE5DC40EC9E7E0512791D328FAA010DCB8]] during bacterial-fungal interaction
Enzyme Classifications	EC 3.1.3.3: phosphoserine phosphatase
Function	control of [[protein\|580011DE5DC40EC9E7E0512791D328FAA010DCB8]] activity
Is essential?	no
Isoelectric point	4.83
Locus Tag	BSU_34110
Molecular weight	45.8694
Name	rsbP
Product	protein serine phosphatase, energy PP2C

RefSeq

Description	Information
Alternative Locus Tag	BSU34110
Description	Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 10632888, 11591687,15342585, 15632289, 16267279, 19948797, 21980452,23524614; Product type e: enzyme
Enzyme Classifications	EC 3.1.3.3: phosphoserine phosphatase
Functions	16.3: Control
	16.6: Maintain
	16.8: Protect
Locus Tag	BSU_34110
Name	rsbP
Title	phosphoserine protein-phosphatase
Type	CDS

BsubCyc

Description	Information
Alternative Name	yvfP
Citation	Avila-Perez M;van der Steen JB;Kort R;Hellingwerf KJ Red light activates the sigmaB-mediated general stress response of Bacillus subtilis via the energy branch of the upstream signaling cascade. J Bacteriol 192(3);755-62 (2010) PUBMED: 19948797
	Bandow JE;Brotz H;Hecker M Bacillus subtilis tolerance of moderate concentrations of rifampin involves the sigma(B)-dependent general and multiple stress response. J Bacteriol 184(2);459-67 (2002) PUBMED: 11751823
	Brigulla M;Hoffmann T;Krisp A;Volker A;Bremer E;Volker U Chill induction of the SigB-dependent general stress response in Bacillus subtilis and its contribution to low-temperature adaptation. J Bacteriol 185(15);4305-14 (2003) PUBMED: 12867438
	Brody MS;Stewart V;Price CW Bypass suppression analysis maps the signalling pathway within a multidomain protein: the RsbP energy stress phosphatase 2C from Bacillus subtilis. Mol Microbiol 72(5);1221-34 (2009) PUBMED: 19432806
	Brody MS;Vijay K;Price CW Catalytic function of an alpha/beta hydrolase is required for energy stress activation of the sigma(B) transcription factor in Bacillus subtilis. J Bacteriol 183(21);6422-8 (2001) PUBMED: 11591687
	Delumeau O;Lewis RJ;Yudkin MD Protein-protein interactions that regulate the energy stress activation of sigma(B) in Bacillus subtilis. J Bacteriol 184(20);5583-9 (2002) PUBMED: 12270815
	Eymann C;Hecker M Induction of sigma(B)-dependent general stress genes by amino acid starvation in a spo0H mutant of Bacillus subtilis. FEMS Microbiol Lett 199(2);221-7 (2001) PUBMED: 11377871
	Holtmann G;Brigulla M;Steil L;Schutz A;Barnekow K;Volker U;Bremer E RsbV-independent induction of the SigB-dependent general stress regulon of Bacillus subtilis during growth at high temperature. J Bacteriol 186(18);6150-8 (2004) PUBMED: 15342585
	Kaneko T;Tanaka N;Kumasaka T Crystal structures of RsbQ, a stress-response regulator in Bacillus subtilis. Protein Sci 14(2);558-65 (2005) PUBMED: 15632289
	Makino M;Kondo S;Kaneko T;Baba S;Hirata K;Kumasaka T Expression, crystallization and preliminary crystallographic analysis of the PAS domain of RsbP, a stress-response phosphatase from Bacillus subtilis. Acta Crystallogr Sect F Struct Biol Cryst Commun 65(Pt 6);559-61 (2009) PUBMED: 19478430
	Nadezhdin EV;Brody MS;Price CW An α/β hydrolase and associated Per-ARNT-Sim domain comprise a bipartite sensing module coupled with diverse output domains. PLoS One 6(9);e25418 (2011) PUBMED: 21980452
	Obuchowski M [Serine-threonine protein phosphatases from Bacillus subtilis]. Postepy Biochem 51(1);95-104 (2005) PUBMED: 16209347
	Ringus DL;Gaballa A;Helmann JD;Wiedmann M;Boor KJ Fluoro-phenyl-styrene-sulfonamide, a novel inhibitor of σB activity, prevents the activation of σB by environmental and energy stresses in Bacillus subtilis. J Bacteriol 195(11);2509-17 (2013) PUBMED: 23524614
	Vijay K;Brody MS;Fredlund E;Price CW A PP2C phosphatase containing a PAS domain is required to convey signals of energy stress to the sigmaB transcription factor of Bacillus subtilis. Mol Microbiol 35(1);180-8 (2000) PUBMED: 10632888
	Zhang S;Haldenwang WG Contributions of ATP, GTP, and redox state to nutritional stress activation of the Bacillus subtilis sigmaB transcription factor. J Bacteriol 187(22);7554-60 (2005) PUBMED: 16267279
Comment	RsbP is a protein-serine phosphatase that responds to energy stress. It dephosphorylates \|FRAME: BSU04710-MONOMER RsbV\|-phosphate in vitro \|CITS: [10632888]\|. RsbP: "regulator of sigma B, phosphatase" \|CITS: [10632888]\| 16.6: Maintain 16.3: Control 16.8: Protect
Description	protein-serine phosphatase
Enzyme Classifications	EC 3.1.3.16: protein-serine/threonine phosphatase
Gene Ontology	GO:0003824 catalytic activity
	GO:0004647 phosphoserine phosphatase activity
	GO:0004721 phosphoprotein phosphatase activity
	GO:0004871 NA
	GO:0007165 signal transduction
	GO:0008152 metabolic process
	GO:0016311 dephosphorylation
	GO:0016787 hydrolase activity
Locus Tag	BSU34110
Molecular weight	46.03
Name	rsbP

Nicolas et al. predictions

Description	Information
Expression neg. correlated with	BSU10600, new_3153832_3153982_c, BSU08779, BSU18140, new_1912142_1913240_c, BSU22250, BSU19430, new_525021_528076_c, BSU18210, BSU07250
Expression pos. correlated with	BSU34100, new_3577620_3577744_c, BSU34820, BSU06340, BSU06330, BSU38860, BSU34060, BSU28590, BSU05620, BSU31500
Highly expressed condition	(BC) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC].
	(Cold) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(ferm) Cells were grown in a synthetic medium (E. Härtig, A. Hartmann, M. Schätzle, A. M. Albertini, D. Jahn, Appl Environ Microbiol 72, 5260, 2006) at 37 °C. For aerobic growth, an overnight culture was used to inoculate 100 ml of the synthetic medium to a starting OD578 of 0.05. The culture was then incubated in a 500 ml baffled flask with shaking at 250 rpm [aero]. Anaerobic growth was carried out (i) in the presence of 10 mM potassium nitrate (nitrate respiration) [nit]; or (ii) in the absence of 10 mM postassium nitrate (fermentative growth) [ferm]. The procedure for anaerobic growth was: medium was inoculated to an OD578 nm of 0.1 in flasks completely filled with medium and sealed with rubber stoppers. They were shaken at 100 rpm to minimize cell aggregation. These cultures were inoculated aerobically with an aerobically grown overnight culture. Anaerobic conditions were achieved in the stoppered flasks after a short time through the consumption of residual oxygen. Cells were harvested during the exponential growth phase.
	(G135) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180].
	(LBGstat) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(LBGtran) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(M0t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90].
	(M40t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90].
	(Sw) Exponentially growing cells were spotted on 1 % agar LB plates and incubated at 37°C. Swarming cells were collected after 16 hours.
Lowely expressed condition	(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(M9stat) Cells were grown in M9 supplemented with glucose (0.3 %) at 37°C with vigorous shaking. The composition of the M9 minimal medium is (per liter): 8.5 g Na2HPO4.2H20, 3 g KH2PO4, 1 g NH4Cl and 0.5 g NaCl. The following solutions were individually sterilized and added (volumes per liter of medium): 1 ml 0.1 M CaCl2.2H2O, 1 ml 1 M MgSO4.7H2O, 1 ml 50 mM Fe-Citrate. Also added was 10 ml of a trace salts solution containing (per liter): 170 mg ZnCl2, 100 mg MnCl2.4H2O, 60 mg CoCl2.6H2O, 60 mg Na2MoO4.2H2O and 43 mg CuCl2.2H2O. Overnight cultures were diluted 2000-fold in pre-warmed M9 medium and samples were harvested during exponential growth [M9exp], at the transition phase [M9tran] and during stationary phase [M9stat].
	(Pyr) A 5 ml aliquot of LB medium was inoculated using frozen culture stocks. After a few hours growth at 37°C, precultures were prepared by inoculating 5 ml of M9 with this LB culture at several different dilutions usually ranging from 500- to 2000-fold. The dilution range was chosen so that one of these precultures had grown to and OD600 of 0.5 - 1.0 after overnight inculation. The chosen M9 medium precultures [at OD600 of 0.5 - 1.0] were used to inoculate 100 mL of M9 medium in 500 mL non-baffled shake flasks to an OD600 of 0.02. Filter-sterilized carbon sources were added separately to the medium M9 at following concentration: D-Glucose 3g/L[Glu], L-Malic acid 4.5g/L[Mal], L-Malic acid + D-Glucose 3 and 2g/L[M+G], D-Fructose 3g/L[Fru], D-Gluconate 4g/L[Glucon], Pyruvate 6g/L[Pyr], Glycerol 6g/L[Gly], Glutamic acid + Succinic acid 2 and 2g/L[G+S]. Where necessary, carbon source solutions were pH neutralized with 4 M NaOH prior to addition to the medium. Cells were harvested during the exponential growth phase.
	(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(T0.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
	(T5.0H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H].
Name	rsbP

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