BSGatlas

bmrA

BSGatlas-gene-4069

BSGatlas

Description	Information
Coordinates	3577745..3579514
Genomic Size	1770 bp
Name	bmrA
Outside Links	SubtiWiki
	BsubCyc
Strand	-
Type	CDS

SubtiWiki

Description	Information
Alternative Name	bmrA
	bmrA
	yvcC
Category	SW 1 Cellular processes
	SW 1.2 Transporters
	SW 1.2.1 ABC transporters
	SW 1.2.1.2 Exporters
	SW 1.2.1.2.1 Efflux of antibiotics
	SW 4 Lifestyles
	SW 4.3 Coping with stress
	SW 4.3.13 Resistance against toxins/ antibiotics
	SW 6 Groups of genes
	SW 6.2 Membrane proteins
Description	multidrug [SW\|ABC transporter] (ATP-binding protein)
Function	multiple antibiotic resistance
Is essential?	no
Isoelectric point	6.47
Locus Tag	BSU_34820
Molecular weight	64.3447
Name	bmrA
Product	multidrug [SW\|ABC transporter] (ATP-binding protein)

RefSeq

Description	Information
Alternative Locus Tag	BSU34820
Description	Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 16405427, 18215075,21077936, 22711831, 24630999; Product type t :transporter
Functions	16.1: Circulate
Locus Tag	BSU_34820
Name	bmrA
Title	efflux transporter (ATP-binding and permeaseprotein)
Type	CDS

BsubCyc

Description	Information
Alternative Name	yvcC
Citation	Browning LM;Lee KJ;Cherukuri PK;Nallathamby PD;Warren S;Jault JM;Xu XN Single Nanoparticle Plasmonic Spectroscopy for Study of the Efflux Function of Multidrug ABC Membrane Transporters of Single Live Cells. RSC Adv 6(43);36794-36802 (2016) PUBMED: 27570617
	Ding F;Lee KJ;Vahedi-Faridi A;Huang T;Xu XH Design and probing of efflux functions of EGFP fused ABC membrane transporters in live cells using fluorescence spectroscopy. Anal Bioanal Chem 400(1);223-35 (2011) PUBMED: 21336797
	Fribourg PF;Chami M;Sorzano CO;Gubellini F;Marabini R;Marco S;Jault JM;Levy D 3D cryo-electron reconstruction of BmrA, a bacterial multidrug ABC transporter in an inward-facing conformation and in a lipidic environment. J Mol Biol 426(10);2059-69 (2014) PUBMED: 24630999
	Lee KJ;Browning LM;Huang T;Ding F;Nallathamby PD;Xu XH Probing of multidrug ABC membrane transporters of single living cells using single plasmonic nanoparticle optical probes. Anal Bioanal Chem 397(8);3317-28 (2010) PUBMED: 20544182
	Mehmood S;Domene C;Forest E;Jault JM Dynamics of a bacterial multidrug ABC transporter in the inward- and outward-facing conformations. Proc Natl Acad Sci U S A 109(27);10832-6 (2012) PUBMED: 22711831
	Wiseman B;Kilburg A;Chaptal V;Reyes-Mejia GC;Sarwan J;Falson P;Jault JM Stubborn Contaminants: Influence of Detergents on the Purity of the Multidrug ABC Transporter BmrA. PLoS One 9(12);e114864 (2014) PUBMED: 25517996
Comment	Mutations that increase transcription and mRNA stability of bmrA lead to increased resistance to the antibiotic cervimycin C \|CITS: [21077936]\|. Annotation from GenBank: Evidence 1a: Function experimentally demonstrated in the studied strain; PubMedId: 16405427, 18215075; Product type t: transporter 16.1: Circulate
Description	efflux transporter (ATP-binding and permease protein)
Enzyme Classifications	EC 3.6.3.44: xenobiotic-transporting ATPase
Gene Ontology	GO:0000166 nucleotide binding
	GO:0005524 ATP binding
	GO:0005886 plasma membrane
	GO:0006810 transport
	GO:0006869 lipid transport
	GO:0008152 metabolic process
	GO:0016020 membrane
	GO:0016021 integral component of membrane
	GO:0016787 hydrolase activity
	GO:0016887 ATPase activity
	GO:0017111 nucleoside-triphosphatase activity
	GO:0034040 lipid-transporting ATPase activity
	GO:0042626 ATPase activity, coupled to transmembrane movement of substances
	GO:0046677 response to antibiotic
	GO:0055085 transmembrane transport
Locus Tag	BSU34820
Molecular weight	64.519
Name	bmrA

Nicolas et al. predictions

Description	Information
Expression neg. correlated with	BSU32950, BSU10600, BSU21310, BSU22250, new_988013_988416_c, BSU26960, new_2245307_2246150_c, BSU26990, BSU13380, BSU26970
Expression pos. correlated with	new_3577620_3577744_c, BSU31500, BSU31490, BSU28590, BSU05620, BSU06330, BSU06340, BSU34110, BSU28920, BSU23750
Highly expressed condition	(BC) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC].
	(BI) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC].
	(C90) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal.
	(Cold) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(LBexp) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(LBGstat) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(LBGtran) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(M0t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90].
	(M40t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90].
	(Sw) Exponentially growing cells were spotted on 1 % agar LB plates and incubated at 37°C. Swarming cells were collected after 16 hours.
Lowely expressed condition	(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(Pyr) A 5 ml aliquot of LB medium was inoculated using frozen culture stocks. After a few hours growth at 37°C, precultures were prepared by inoculating 5 ml of M9 with this LB culture at several different dilutions usually ranging from 500- to 2000-fold. The dilution range was chosen so that one of these precultures had grown to and OD600 of 0.5 - 1.0 after overnight inculation. The chosen M9 medium precultures [at OD600 of 0.5 - 1.0] were used to inoculate 100 mL of M9 medium in 500 mL non-baffled shake flasks to an OD600 of 0.02. Filter-sterilized carbon sources were added separately to the medium M9 at following concentration: D-Glucose 3g/L[Glu], L-Malic acid 4.5g/L[Mal], L-Malic acid + D-Glucose 3 and 2g/L[M+G], D-Fructose 3g/L[Fru], D-Gluconate 4g/L[Glucon], Pyruvate 6g/L[Pyr], Glycerol 6g/L[Gly], Glutamic acid + Succinic acid 2 and 2g/L[G+S]. Where necessary, carbon source solutions were pH neutralized with 4 M NaOH prior to addition to the medium. Cells were harvested during the exponential growth phase.
	(S3) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(Salt) Cells were grown in Spizizen’s minimal medium (SMM) at 37 °C with vigorous shaking. Salt was added, to a final concentration of 0.4 M to an exponentially growing culture of cells at OD500 of 0.4. Samples were harvested before [SMM] and 10 minutes after [Salt] NaCl addition.
Name	bmrA

KEGG Pathways

Description	Information
Pathway	beta-Lactam resistance (ko01501)
	ABC transporters (ko02010)

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