secA
BSGatlas-gene-4120
BSGatlas
Description | Information |
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Coordinates | 3628310..3630835 |
Genomic Size | 2526 bp |
Name | secA |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | - |
Type | CDS |
SubtiWiki
Description | Information |
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Alternative Name | div |
div-341 | |
secA | |
secA | |
ts-341 | |
Category | SW 3 Information processing |
SW 3.3 Protein synthesis, modification and degradation | |
SW 3.3.5 Protein secretion | |
SW 6 Groups of genes | |
SW 6.1 Essential genes | |
SW 6.2 Membrane proteins | |
Description | preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel |
Function | protein secretion |
Is essential? | yes |
Isoelectric point | 5.34 |
Locus Tag | BSU_35300 |
Molecular weight | 95.3335 |
Name | secA |
Product | preprotein translocase subunit (ATPase) |
RefSeq
Description | Information |
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Alternative Locus Tag | BSU35300 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 9880811, 10074074,10481054, 10559168, 10713435, 11021932, 12682299,14621992, 16243836, 18923516, 23167435, 24592260,26607006, 27336478; Product type e: enzyme |
Functions | 16.1: Circulate |
Locus Tag | BSU_35300 |
Name | secA |
Title | translocase binding subunit (ATPase) |
Type | CDS |
BsubCyc
Description | Information |
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Alternative Name | div |
div-341 | |
ts-341 | |
Citation | Cui P;Li X;Zhu M;Wang B;Liu J;Chen H Design, synthesis and antibacterial activities of thiouracil derivatives containing acyl thiourea as SecA inhibitors. Bioorg Med Chem Lett (2016) PUBMED: 28041832 |
Dajkovic A;Hinde E;MacKichan C;Carballido-Lopez R Dynamic Organization of SecA and SecY Secretion Complexes in the B. subtilis Membrane. PLoS One 11(6);e0157899 (2016) PUBMED: 27336478 | |
Halbedel S;Kawai M;Breitling R;Hamoen LW SecA is required for membrane targeting of the cell division protein DivIVA in vivo. Front Microbiol 5;58 (2014) PUBMED: 24592260 | |
Kim DM;Zheng H;Huang YJ;Montelione GT;Hunt JF ATPase active-site electrostatic interactions control the global conformation of the 100 kDa SecA translocase. J Am Chem Soc 135(8);2999-3010 (2013) PUBMED: 23167435 | |
Milenkovic S;Bondar AN Mechanism of conformational coupling in SecA: Key role of hydrogen-bonding networks and water interactions. Biochim Biophys Acta 1858(2);374-85 (2016) PUBMED: 26607006 | |
Song Y;Nikoloff JM;Zhang D Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis. J Microbiol Biotechnol 25(7);963-77 (2015) PUBMED: 25737123 | |
Wowor AJ;Yan Y;Auclair SM;Yu D;Zhang J;May ER;Gross ML;Kendall DA;Cole JL Analysis of SecA dimerization in solution. Biochemistry 53(19);3248-60 (2014) PUBMED: 24786965 | |
Yang CK;Lu CD;Tai PC Differential expression of secretion machinery during bacterial growth: SecY and SecF decrease while SecA increases during transition from exponential phase to stationary phase. Curr Microbiol 67(6);682-7 (2013) PUBMED: 23852076 | |
Zimmer J;Rapoport TA Conformational flexibility and peptide interaction of the translocation ATPase SecA. J Mol Biol 394(4);606-12 (2009) PUBMED: 19850053 | |
Comment | 16.1: Circulate |
Description | translocase binding subunit (ATPase) |
Gene Ontology | GO:0000166 nucleotide binding |
GO:0003676 nucleic acid binding | |
GO:0004386 helicase activity | |
GO:0005524 ATP binding | |
GO:0005737 cytoplasm | |
GO:0005886 plasma membrane | |
GO:0006605 protein targeting | |
GO:0006810 transport | |
GO:0008152 metabolic process | |
GO:0015031 protein transport | |
GO:0016020 membrane | |
GO:0017038 protein import | |
GO:0046872 metal ion binding | |
GO:0065002 intracellular protein transmembrane transport | |
Locus Tag | BSU35300 |
Molecular weight | 95.531 |
Name | secA |
Nicolas et al. predictions
Description | Information |
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Expression neg. correlated with | BSU27420, BSU22040, BSU22050, BSU21310, new_2245307_2246150_c, BSU10610, BSU17820, new_3153832_3153982_c, BSU24480, BSU18290 |
Expression pos. correlated with | new_3628241_3628309_c, BSU35290, BSU07370, BSU30390, BSU40410, BSU00320, BSU30400, new_3195466_3195557_c, BSU35670, BSU10620 |
Highly expressed condition | (BC) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC]. |
(BI) Cultures were inoculated from frozen glycerol stocks and grown overnight in LB at 37°C. These cultures were thendiluted, plated onto LB plates, and incubated for 16 h at 37°C. Cells were harvested from plates containing individual colonies [BI] andfrom plates with confluen growth [BC]. | |
(Cold) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(G150) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180]. | |
(LBGtran) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(M0t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(M40t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(Sw) Exponentially growing cells were spotted on 1 % agar LB plates and incubated at 37°C. Swarming cells were collected after 16 hours. | |
(T0.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Lowely expressed condition | (BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(LoTm) Cells were grown in Spizizen’s minimal medium (SMM) (C. Anagnostopoulos, J. Spizizen, J Bacteriol 81, 741, May, 1961) with vigorous agitation. The control culture was grown at 37 °C [SMMPr]. For growth at high or low temperatures, pre-cultures were grown at 37 °C, diluted to an OD578nm of 0.1 and subsequently transferred to 51 °C [HiTm] and 16 °C [LoTm], respectively. For the growth at high salinity, the salinity of the medium was adjusted by adding NaCl (5 M stock solution) to produce a final concentration of 1.2 M [HiOs]. | |
(MG+150) A culture of LB medium was inocualted from a frozen glycerol stock of B. subtilis. After few hours at 37oC when the culture was growing exponentially, this culture was used to inoculate M9 minimal medium at several different dilutions usually in the range of 500- to 2000-fold. The dilution range was chosen to ensure that at least one of these M9 precultures had reached an OD600 between 0.5 - 1.0 after overnight incubation. These precultures were then used to inoculate 2.5 L of M9 medium in a 3.1 L KLF bioreactor (Bioengineering AG, Wald, Switzerland) to a starting OD600 of 0.03 – 0.05. Condiions in the bioreactor were rigorously controlled as follows: temperature was controlled at 37 °C; the pH was maintained at exactly 7.2 by automatic titration with 2.0 M KOH and 2.0 M H2SO4, and the dissolved oxygen tension was maintained above 50%. In each nutritional shift experiment cells were grown on the single substrate until the OD600 reached 0.50, at which point the second substrate was added instantaneously (4 g/L L-malate or 3 g/L glucose). The nutrient shifts performed were from glucose to glucose+malate [GM] and from malate to malate+glucose [MG] (Buescher et al., accompanying paper). Cell growth during the course was monitored throughout the experiment by measuring OD600. | |
(S3) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
Name | secA |
KEGG Pathways
Description | Information |
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Pathway | Quorum sensing (ko02024) |
Protein export (ko03060) | |
Bacterial secretion system (ko03070) |