rsbR
BSGatlas-gene-567
BSGatlas
Description | Information |
---|---|
Coordinates | 519408..520232 |
Genomic Size | 825 bp |
Name | rsbR |
Outside Links | SubtiWiki |
BsubCyc | |
Strand | + |
Type | CDS |
SubtiWiki
Description | Information |
---|---|
Alternative Name | rsbR |
rsbR | |
rsbRA | |
ycxR | |
Category | SW 3 Information processing |
SW 3.4 Regulation of gene expression | |
SW 3.4.1 Sigma factors and their control | |
SW 3.4.1.2 Control of sigma factors | |
SW 6 Groups of genes | |
SW 6.4 Phosphoproteins | |
SW 6.4.6 Phosphorylation on a Thr residue | |
Description | [SW|stressosome] sensor protein, control of [[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8]] activity |
Function | control of [[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8]] activity |
Is essential? | no |
Isoelectric point | 4.73 |
Locus Tag | BSU_04670 |
Molecular weight | 30.8983 |
Name | rsbR |
Product | activator of [[protein|RsbT ]]kinase activity, [SW|stressosome] sensor protein |
RefSeq
Description | Information |
---|---|
Alternative Locus Tag | BSU04670 |
Description | Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 15312768, 15583165,16963570, 17158665, 11157946, 20019076, 21362065,22287516, 23320651, 28271471; Product type f: factor |
Functions | 16.3: Control |
16.8: Protect | |
Locus Tag | BSU_04670 |
Name | rsbRA |
Title | component of the anxiosome (stressosome);positive regulation of sigma(B) activity in response tosalt and heat stress |
Type | CDS |
BsubCyc
Description | Information |
---|---|
Alternative Name | rsbR |
ycxR | |
Citation | Eymann C;Schulz S;Gronau K;Becher D;Hecker M;Price CW In vivo phosphorylation patterns of key stressosome proteins define a second feedback loop that limits activation of Bacillus subtilis σ(B). Mol Microbiol 80(3);798-810 (2011) PUBMED: 21362065 |
Gaidenko TA;Bie X;Baldwin EP;Price CW Substitutions in the presumed sensing domain of the Bacillus subtilis stressosome affect its basal output but not response to environmental signals. J Bacteriol 193(14);3588-97 (2011) PUBMED: 21602359 | |
Gaidenko TA;Bie X;Baldwin EP;Price CW Two surfaces of a conserved interdomain linker differentially affect output from the RST sensing module of the Bacillus subtilis stressosome. J Bacteriol 194(15);3913-21 (2012) PUBMED: 22609918 | |
Jurk M;Schramm P;Schmieder P The blue-light receptor YtvA from Bacillus subtilis is permanently incorporated into the stressosome independent of the illumination state. Biochem Biophys Res Commun 432(3);499-503 (2013) PUBMED: 23416074 | |
Liebal UW;Millat T;Marles-Wright J;Lewis RJ;Wolkenhauer O Simulations of stressosome activation emphasize allosteric interactions between RsbR and RsbT. BMC Syst Biol 7;3 (2013) PUBMED: 23320651 | |
van der Steen JB;Avila-Perez M;Knippert D;Vreugdenhil A;van Alphen P;Hellingwerf KJ Differentiation of function among the RsbR paralogs in the general stress response of Bacillus subtilis with regard to light perception. J Bacteriol 194(7);1708-16 (2012) PUBMED: 22287516 | |
Comment | 16.8: Protect 16.3: Control |
Description | component of the piezosome (stressosome); positive regulation of sigma(B) activity in response to salt and heat stress |
Locus Tag | BSU04670 |
Molecular weight | 31.05 |
Name | rsbRA |
Nicolas et al. predictions
Description | Information |
---|---|
Expression neg. correlated with | new_3316213_3316329_c, BSU19259, new_2098330_2098837, BSU12110, new_1211286_1211370, new_1159082_1159149, new_2298463_2299409, BSU13360, BSU32270, BSU06920 |
Expression pos. correlated with | BSU04690, BSU04680, BSU04700, BSU14270, BSU06060, BSU06070, BSU24530, new_3809980_3810619_c, BSU33221, BSU14260 |
Highly expressed condition | (dia15) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15]. |
(dia5) Diamide was added to an exponentially growing culture (OD600 approx. 0.6) at a sub-lethal concentration(0.5 mM) and growth continued at 37°C with vigorous shaking. Samples were collected 0, 5 and 15 minutes after diamide addition [dia0, dia5 and dia15]. | |
(Diami) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(H2O2) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(Heat) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha]. | |
(LBtran) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle . | |
(M40t45) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(Mt0) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90]. | |
(Paraq) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition | |
(Salt) Cells were grown in Spizizen’s minimal medium (SMM) at 37 °C with vigorous shaking. Salt was added, to a final concentration of 0.4 M to an exponentially growing culture of cells at OD500 of 0.4. Samples were harvested before [SMM] and 10 minutes after [Salt] NaCl addition. | |
Lowely expressed condition | (B60) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. |
(BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60]. | |
(S3) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S4) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments. | |
(T0.30H) Anon-sporulating B. subtilis strain was grown in a modified M9 medium in batch culture (T. Hardiman, K. Lemuth, M. A. Keller, M. Reuss, M. Siemann-Herzberg, J Biotechnol 132, 359, Dec 1, 2007). Glucose was exhausted when the culture reached an OD600 of approx. 10 and this was designated T0 [T0.0H]. 7 samples were harvested at various times before glucose exhaustion [T-5.40H to T-0.40H] and 10 samples at various times after glucose exhaustion [T0.30H to T5.0H]. | |
Name | rsbRA |