BSGatlas

dgkB

BSGatlas-gene-834

BSGatlas

Description	Information
Coordinates	736436..737347
Genomic Size	912 bp
Name	dgkB
Outside Links	SubtiWiki
	BsubCyc
Strand	+
Type	CDS

SubtiWiki

Description	Information
Alternative Name	dgkB
	dgkB
	yerQ
Category	SW 1 Cellular processes
	SW 1.1 Cell envelope and cell division
	SW 1.1.1 Cell wall synthesis
	SW 1.1.1.3 Biosynthesis of lipoteichoic acid
	SW 2 Metabolism
	SW 2.6 Additional metabolic pathways
	SW 2.6.1 Biosynthesis of cell wall components
	SW 2.6.1.2 Biosynthesis of lipoteichoic acid
Description	diacylglycerol kinase
Enzyme Classifications	EC 2.7.1.107: diacylglycerol kinase (ATP)
Function	biosynthesis of lipoteichoic acid
Is essential?	no
Isoelectric point	5.64
Locus Tag	BSU_06720
Molecular weight	33.1835
Name	dgkB
Product	diacylglycerol kinase

RefSeq

Description	Information
Alternative Locus Tag	BSU06720
Description	Evidence 1a: Function from experimental evidencesin the studied strain; PubMedId: 10220166, 12682299,17535816, 22224599, 24700682; Product type e: enzyme
Enzyme Classifications	EC 2.7.1.107: diacylglycerol kinase (ATP)
Functions	16.2: Construct biomass (Anabolism)
	16.6: Maintain
	16.8: Protect
Locus Tag	BSU_06720
Name	dagK
Title	diacylglycerol kinase
Type	CDS

BsubCyc

Description	Information
Alternative Name	dagK
	yerQ
Citation	Kobayashi K;Ehrlich SD;Albertini A;Amati G;Andersen KK;Arnaud M;Asai K;Ashikaga S;Aymerich S;Bessieres P;Boland F;Brignell SC;Bron S;Bunai K;Chapuis J;Christiansen LC;Danchin A;Debarbouille M;Dervyn E;Deuerling E;Devine K;Devine SK;Dreesen O;Errington J;Fillinger S;Foster SJ;Fujita Y;Galizzi A;Gardan R;Eschevins C;Fukushima T;Haga K;Harwood CR;Hecker M;Hosoya D;Hullo MF;Kakeshita H;Karamata D;Kasahara Y;Kawamura F;Koga K;Koski P;Kuwana R;Imamura D;Ishimaru M;Ishikawa S;Ishio I;Le Coq D;Masson A;Mauel C;Meima R;Mellado RP;Moir A;Moriya S;Nagakawa E;Nanamiya H;Nakai S;Nygaard P;Ogura M;Ohanan T;O'Reilly M;O'Rourke M;Pragai Z;Pooley HM;Rapoport G;Rawlins JP;Rivas LA;Rivolta C;Sadaie A;Sadaie Y;Sarvas M;Sato T;Saxild HH;Scanlan E;Schumann W;Seegers JF;Sekiguchi J;Sekowska A;Seror SJ;Simon M;Stragier P;Studer R;Takamatsu H;Tanaka T;Takeuchi M;Thomaides HB;Vagner V;van Dijl JM;Watabe K;Wipat A;Yamamoto H;Yamamoto M;Yamamoto Y;Yamane K;Yata K;Yoshida K;Yoshikawa H;Zuber U;Ogasawara N Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100(8);4678-83 (2003) PUBMED: 12682299
	Matsuoka S;Hashimoto M;Kamiya Y;Miyazawa T;Ishikawa K;Hara H;Matsumoto K The Bacillus subtilis essential gene dgkB is dispensable in mutants with defective lipoteichoic acid synthesis. Genes Genet Syst 86(6);365-76 (2011) PUBMED: 22451476
	Petersohn A;Antelmann H;Gerth U;Hecker M Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis. Microbiology 145 ( Pt 4);869-80 (1999) PUBMED: 10220166
Comment	DgkB is the Bacillus diacylglycerol kinase; it only phosphorylates diacylglycerols and is more active toward substrates with long acyl chains \|CITS: [17535816]\|. Downregulation of dgkB expression leads to accumulation of diacylglycerol and cessation of lipoteichoic acid synthesis \|CITS: [17535816]\|. The lethal phenotype is suppressed by disruption of ltaS or ltaSA \|CITS: [22451476]\|.
Description	diacylglycerol kinase
Enzyme Classifications	EC 2.7.1.107: diacylglycerol kinase (ATP)
Gene Ontology	GO:0000166 nucleotide binding
	GO:0003951 NAD+ kinase activity
	GO:0004143 diacylglycerol kinase activity
	GO:0005524 ATP binding
	GO:0006629 lipid metabolic process
	GO:0007205 protein kinase C-activating G protein-coupled receptor signaling pathway
	GO:0008152 metabolic process
	GO:0008654 phospholipid biosynthetic process
	GO:0016301 kinase activity
	GO:0016310 phosphorylation
	GO:0016740 transferase activity
	GO:0046872 metal ion binding
	GO:0070395 lipoteichoic acid biosynthetic process
Locus Tag	BSU06720
Molecular weight	33.337
Name	dgkB

Nicolas et al. predictions

Description	Information
Expression neg. correlated with	BSU09889, BSU03780, BSU29260, BSU23460, BSU23470, BSU23450, new_2076113_2076205, new_2996680_2996979, BSU33640, BSU02340
Expression pos. correlated with	BSU06730, BSU29900, BSU27620, BSU27360, new_737348_737602, new_3860876_3860997_c, BSU00030, BSU37610, BSU01580, BSU27350
Highly expressed condition	(C30) Cellsgrown overnight on LB agar plates at 30°Cwere harvested and used to inoculate pre-warmed minimal medium at OD600 of 0.5 (D. Dubnau, R. Davidoff-Abelson, J Mol Biol 56, 209, Mar 14, 1971). After growth at 37°C with vigorous shaking, cells were diluted ten times in fresh pre-warmed minimal medium and samples were harvested after a period of 30 minutes [C30] , i.e. before maximal induction of competence, and after a period of 90 minutes [C90], i.e. when competence induction was maximal.
	(Cold) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(G150) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180].
	(G180) Purified spores were obtained by growing cells in DSM medium (P. Schaeffer, J. Millet, J. P. Aubert, Proc Natl Acad Sci U S A 54, 704, Sep, 1965) at 37°C for 48 hours after which they were washed ten times in ice cold distilled waterover a period of 5 days. Purified spores were heat activated at 70°C in Tris 10 mM pH8.4 and germination was initiated by the addition of L-alanine 10 mM (A. Moir, J Bacteriol 146, 1106, Jun, 1981). After incubation for one hour at 37°C, the culture was diluted with an equal volume of 2X LBmedium and germinating cells were harvested at 135, 150 or 180 minutes after addition of L-alanine [G135, G150 and G180].
	(H2O2) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition
	(Heat) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(LBGexp) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(LBGstat) Cells were grown in Luria-Bertani medium (Sigma) supplemented with glucose 0.3 % [LBG] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(Oxctl) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition
	(Paraq) Cells were grown in LB medium at 37°C. At OD540 of 0.3, the culture were divided into four subcultures and diamide 0.6 mM [Diami], paraquat 0.4 mM [Paraq], H2O2 0.1mM [H2O2] or no oxidative drug [Oxctl] were added to the medium. Samples were taken 10 minutes after addition
Lowely expressed condition	(B36) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60].
	(BT) A fresh colony grown on an LB plate was used to inoculate 10 ml of LB and grown for 10 hoursat 30°C. This culture wasused to inoculate 10 ml of MSgg medium (S.S. Branda et al., J Bacteriol 186, 3970, Jun, 2004) and incubated with vigorous shaking. The cultures in MSgg were diluted to the same extent in 96 wells microtiterplates (5 μl for 1.5 ml of medium) and incubated without shaking at 30°C. Cells from the control cultures were harvested after 24 hours of incubation [BT]. Biofilms were harvested from 96 well plates after incubation for 36 hours [B36] and 60 hours [B60].
	(Etha) Cells were grown in a synthetic medium (J. Stülke, R. Hanschke, M. Hecker, J Gen Microbiol 139, 2041, Sep, 1993) with 0.2 % glucose as carbon source (Belitsky Minimal Medium/BMM) at 37 °C with vigorous shaking. Stress was applied to exponentially growing cultures at OD500nm of 0.4. Samples were harvested before stress [BMM]; after a rapid temperature up-shift from 37 °C to 48 °C [Heat]; after a temperature down-shift from 37 °C to 18 °C [Cold]. Ethanol stress was imposed by adding ethanol to a final concentration of 4 % (v/v) and cells were harvested 10 minutes after ethanol addition [Etha].
	(LBstat) Cells were grown in Luria-Bertani medium (Sigma) [LB] at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 2000-fold in fresh pre-warmed medium and samples were collected during the exponential [exp], transition [tran] and stationary [stat] phases of the growth cycle .
	(M40t90) Cells were grown in LB medium at 37°C with vigorous shaking. An exponentially growing culture (O.D.600 approx. 0.25) was divided: one culture acted as the control [no mitomycin C , M0] while mitomycin was added to the second culture to a final concentration of 40 ng/ml [mitomycin, M40]. Samples were harvested at 0, 45 and 90 minutes after mitomycin addition [t0, t45 and t90].
	(S2) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S5) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S6) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S7) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
	(S8) Cells were grown in CH medium at 37°C and sporulation was induced by resuspension in warm sporulation medium as described by Sterlini and Mandelstam (J. M. Sterlini, J. Mandelstam, Biochem J 113, 29, Jun, 1969). The initiation of sporulation was designated T0, the time of resuspension. Samples were harvested at hourly intervals for 6 hours [S0 to S6] for the first set of experiments and for 8 hours [S0 to S8] for a second set of experiments.
Name	dgkB

KEGG Pathways

Description	Information
Pathway	Glycerolipid metabolism (ko00561)
	Glycerophospholipid metabolism (ko00564)
	Metabolic pathways (ko01100)
	Biosynthesis of secondary metabolites (ko01110)

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